Methylbenzene recycling method applied to sulfosalicylic acid method for determination of proline contents inside plants

A technology of sulfosalicylic acid and plant body, which is applied in chemical instruments and methods, color/spectral characteristic measurement, water/sewage multi-stage treatment, etc. It can solve the problems of environmental pollution, large amount of toluene waste liquid, and high test cost. , to achieve the effect of reducing pollution, large processing capacity and improving utilization rate

Inactive Publication Date: 2015-06-10
INNER MONGOLIA UNIV FOR THE NATITIES
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Problems solved by technology

[0003] The present invention aims to solve the technical problems that the amount of toluene waste liquid is large when measuring the proline content in plants by the current sulfosalicylic acid method, the direc

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  • Methylbenzene recycling method applied to sulfosalicylic acid method for determination of proline contents inside plants
  • Methylbenzene recycling method applied to sulfosalicylic acid method for determination of proline contents inside plants

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specific Embodiment approach 1

[0024] Specific embodiment one: present embodiment is a kind of method for recycling the toluene that is applied to the proline content in plant body by sulfosalicylic acid method, specifically carries out according to the following steps:

[0025] 1. Decolorization: add activated carbon to the waste liquid of toluene that uses the sulfosalicylic acid method to determine the proline content in plants, and shake it for 2 minutes with an oscillator to obtain solution I; The volume of the toluene waste liquid of proline content and the mass ratio of gac are 1mL:0.01g;

[0026] 2. Filtration: The solution I obtained in step 1 is filtered once with a medium-speed qualitative filter paper to obtain a toluene recovery solution.

[0027] Advantages of this embodiment:

[0028] 1. The present invention can reduce the pollution of the toluene waste liquid applied to the sulfosalicylic acid method to measure the proline content in plants to the environment, improve the utilization rate ...

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Abstract

The invention relates to a methylbenzene recycling method applied to a sulfosalicylic acid method for determination of proline contents inside plants. The purpose of the invention is to solve the technical problems that a large amount of methylbenzene waste liquor is produced in the sulfosalicylic acid method for determination of proline contents inside plants, the direct pouring of the waste liquor results in serious pollution to the environment and the testing cost is high. The recycling method comprises a decoloring step and a filtering step. The recycling method has the advantages of reducing the pollution of the methylbenzene waste liquor in the sulfosalicylic acid method for determination of proline contents inside plants to the environment, improving the utilization rate of organic solvent and reducing the testing cost, and has the characteristics of being simple in operation, high in processing capacity and easy in manual control and the like; the recycling method has certain application value to the physiological characteristics of plants; the methylbenzene recovery rate is 85%; the recycling times of active carbon is eight; and the recycling method is applicable to treatment of a large amount of samples.

Description

technical field [0001] The invention relates to a method for recovering and utilizing toluene used in the determination of proline content in plants by a sulfosalicylic acid method. technical background [0002] Under adversity conditions (drought, saline-alkali, heat, cold, freezing), the content of proline (Pro) in plants increases significantly. The content of proline in plants reflects the stress resistance of plants to a certain extent. The determination of plant proline content is a common method in plant physiology experiments, and is widely used in various plant scientific researches such as crops, pastures, horticultural plants, and garden plants. The sulfosalicylic acid method is a common method for determining the proline content in plants. The amount of toluene waste liquid produced in the laboratory is large, and it will seriously pollute the environment if it is directly poured out, and the test cost is high. Contents of the invention [0003] The present i...

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Application Information

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IPC IPC(8): C02F9/02G01N21/78G01N21/31
Inventor 张玉霞于华荣郭园周立业包金花王乐
Owner INNER MONGOLIA UNIV FOR THE NATITIES
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