Nodulisporium viridian E and application thereof
A technology of chlortetracycline and polysporidium, which is applied in the field of natural product medicine to achieve the effect of significant anti-senile dementia activity
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Embodiment 1
[0017] Example 1 Large-scale fermentation of Arthrobacter sp. 65-12-7-1 and its sample pretreatment method
[0018] (1) Polyarterial fungus 65-12-7-1 is activated by PDA slope and inoculated into PDB medium, at 25℃ at 200r.min -1 The seed solution was prepared by shaking culture for 5 days, and then inoculated into 165 Erlenmeyer flasks (500 mL) containing PDB medium according to the inoculum amount of 5%. -1 After shaking for 12 days, the fermentation broth was obtained. The PDB medium is composed of the following components in a weight-volume ratio: potato 200g / L, glucose 20g / L, and purified water 1L.
[0019] (2) Adding ethyl acetate to the fermented product for immersion extraction twice, and concentrating the extract to dryness under reduced pressure to obtain a crude extract (11.5 g).
Embodiment 2
[0020] Example 2 Preparation of Nodularia polysporum chloromycin E
[0021] The crude extract was passed through a silica gel column and eluted with cyclohexane and methanol to obtain cyclohexane part C (3.8g) and methanol part M (5.6g); then the methanol part M was subjected to medium and low pressure ODS column chromatography, followed by The methanol-water gradient elution with volume ratios of 30:70, 50:50, 70:30, 90:10 and 100:0 yielded 5 fractions (M1, M2, M3, M4, M5); then the volume ratio was 50 The fraction M2 (1.8g) obtained by elution with :50 methanol-water was subjected to medium and low pressure liquid phase ODS column chromatography, followed by volume ratio 30:70, 35:65, 40:60, 45:55, 50:50, 55:45, 100:0 methanol-water gradient elution, M2a, M2b, M2c, M2d, M2e, M2f, M2g, M2h, M2i, M2j and M2k a total of 11 sub-fractions were obtained. The sub-fraction M2a (105 mg) obtained by eluting methanol-water with a volume ratio of 35:65 was prepared by reversed-phase HPLC,...
Embodiment 3
[0026] The compound of Example 3 improves the learning and memory activity of senile dementia flies
[0027] (1) Cultivation of senile dementia fruit flies
[0028] w 1118 (isoCJ1) As the control background fruit fly of the experiment, it is abbreviated as "2U". Successfully transferred to pathogenic Aβ 42 The protein of Drosophila is (UAS-Aβ 42 ; Abbreviated as "H29.3"). This strain of Drosophila was crossed with the whole brain expressing Gal4 promoter Drosophila to obtain elav-GAL4 c155 (P35) and Aβ 42 Of the fruit fly strain.
[0029] (2) Administration of Drosophila
[0030] The experiment set up three groups of healthy fruit flies drug-free control, disease fruit flies drug-free control and disease fruit flies administration.
[0031] The parents of all tested fruit flies were raised and reproduced in a house with a constant temperature of 24°C and a constant humidity of 42% RH (Relative humidity). On the first day after the emergence of the fruit flies, the control group, the ...
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