Carbic acid a and its application in the preparation of drugs for the prevention and treatment of neurodegenerative diseases
A neurodegenerative and corticosteroid technology, applied in the field of natural medicine, can solve the problems of limited source of autologous nerves and many donor sites, and achieve the effect of significant anti-senile dementia activity.
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Embodiment 1
[0025] Example 1 A large amount of fermentation of the fungus 71-10-1-1 of the genus Charcoa and its sample pretreatment method
[0026] (1) The fungus 71-10-1-1 of the genus Anthracum was activated on a PDA slant and then inoculated in PDB medium, at 25°C at 200r.min -1 Shake culture for 5 days to prepare seed solution, then inoculate 20 Erlenmeyer flasks with rice culture medium according to 5% inoculation amount, and culture at 25°C for 49 days to obtain fermented product. The rice medium is composed of the following components: rice 70g / bottle, purified water 105L / bottle.
[0027] (2) The fermented product was added to ethyl acetate for soaking and extraction three times, and the extract was concentrated to dryness under reduced pressure to obtain a crude extract (43.2 g).
Embodiment 2
[0028] The preparation of embodiment 2 carbic acid A
[0029] The crude extract was passed through a silica gel column and eluted with cyclohexane and methanol to obtain a cyclohexane part C (12.5 g) and a methanol part w (21.9 g); Four fractions (w1, w2, w3, w4) were obtained by methanol-water gradient elution with volume ratios of 20:80, 50:50, 70:30 and 100:0; The obtained sub-fraction w2 (5.1g) was subjected to middle and low pressure liquid phase ODS column chromatography, and the volumes of 10:90, 20:80, 25:75, 30:70, 35:65, 40:60, 45: 55, 50:50, 100:0 methanol-water gradient elution, to obtain w2-1, w2-2, w2-3, w2-4, w2-5, w2-6, w2-7, w2-8, w2-9, w2-10 and w2-11 have a total of 11 sub-fractions. The sub-fraction w2-6 (372.5 mg) obtained by eluting with methanol-water with a volume of 30:70 was subjected to silica gel column chromatography, and was sequentially washed with volumes of 100:0, 97:3, 95:5, 93:7, 9:1, 8:2, 0:100 oxyform-methanol gradient elution to get w2-...
Embodiment 3
[0035] Example 3 Carbolic acid A improves the learning and memory ability of senile dementia Drosophila and its activity test method
[0036] (1) Cultivation of Alzheimer's Drosophila
[0037] w 1118 (isoCJ1) was used as the control group of the experiment, the background Drosophila, abbreviated as "2U". Successful transfer into pathogenic Aβ 42 The Drosophila protein is (UAS-Aβ 42 ; abbreviated as "h29.3"). This strain of Drosophila was crossed with Drosophila expressing the Gal4 promoter in the whole brain to obtain elav-GAL4 c155 (P35) and Aβ 42 Drosophila strains.
[0038] (2) Administration of Alzheimer's Drosophila
[0039] Three groups were set up in the experiment: healthy fruit flies without drug control, diseased fruit flies without drug control and diseased fruit flies with administration.
[0040] The parents of all tested fruit flies were reared and bred in a fly house with a constant temperature of 24° C. and a constant humidity of 42% RH (Relative humidi...
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