Polynosporin e gliomycin e and its application
A green glomycin, compound technology, applied in the field of natural product medicine, to achieve significant anti-Alzheimer's effect
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Embodiment 1
[0017] Example 1 Mass fermentation of Arthrosporum fungus 65-12-7-1 and its sample pretreatment method
[0018] (1) Polyarthrospora fungus 65-12-7-1 was inoculated in PDB medium after being activated on PDA slant, at 25°C at 200r.min -1 Shake culture for 5 days to prepare seed solution, then inoculate 165 Erlenmeyer flasks (500mL) with PDB medium according to 5% inoculum size, at 25°C at 200r.min -1 Under the shaking culture for 12 days, the fermentation broth was obtained. The PDB medium is composed of the following components in weight to volume ratio: potatoes 200g / L, glucose 20g / L, and purified water 1L.
[0019] (2) The fermented product was added to ethyl acetate for soaking and extraction twice, and the extract was concentrated to dryness under reduced pressure to obtain a crude extract (11.5 g).
Embodiment 2
[0020] The preparation of embodiment 2 polyarthrocyanidin E
[0021] The crude extract was passed through a silica gel column and eluted with cyclohexane and methanol to obtain cyclohexane part C (3.8g) and methanol part M (5.6g); then the methanol part M was subjected to medium and low pressure ODS column chromatography, followed by The methanol-water gradient elution with the volume ratio of 30:70, 50:50, 70:30, 90:10 and 100:0 obtained 5 fractions (M1, M2, M3, M4, M5); : 50 methanol-water elution obtained sub-fraction M2 (1.8g) through medium and low pressure liquid ODS column chromatography, followed by volume ratio 30: 70, 35: 65, 40: 60, 45: 55, 50: 50, Eleven sub-fractions of M2a, M2b, M2c, M2d, M2e, M2f, M2g, M2h, M2i, M2j and M2k were obtained by methanol-water gradient elution of 55:45 and 100:0. Sub-fraction M2a (105 mg) obtained by eluting with methanol-water in a volume ratio of 35:65 was prepared by reverse phase HPLC using acetonitrile-water (18:82, v / v) at a f...
Embodiment 3
[0026] Example 3 compound improves the learning and memory activity test method of senile dementia Drosophila
[0027] (1) Cultivation of Alzheimer's Drosophila
[0028] w 1118 (isoCJ1) was used as the control group of the experiment, the background Drosophila, abbreviated as "2U". Successful transfer into pathogenic Aβ 42 The Drosophila protein is (UAS-Aβ 42 ; abbreviated as "H29.3"). This strain of Drosophila was crossed with Drosophila expressing the Gal4 promoter in the whole brain to obtain elav-GAL4 c155 (P35) and Aβ 42 Drosophila strains.
[0029] (2) Administration of Alzheimer's Drosophila
[0030] Three groups were set up in the experiment: healthy fruit flies without drug control, diseased fruit flies without drug control and diseased fruit flies with administration.
[0031] The parents of all tested fruit flies were reared and bred in a fly house with a constant temperature of 24° C. and a constant humidity of 42% RH (Relative humidity). On the first day af...
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