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Binary product fermentation method and two kinds of fermentation products thereof

A fermentation method and product technology, applied in the field of prevention and control of plant diseases and insect pests, neimamicin and its fermentation impurities, can solve the problems of limited effect of neimamicin, lack of research on neimamicin fermentation, etc., achieve dilution of preparation costs, facilitate Promoted app performance

Active Publication Date: 2015-06-10
河北奥思德环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In summary, due to the limited effect of direct use of naimamectin itself, it is mainly used as a synthetic intermediate of moxictin, so the prior art focuses on obtaining high-purity naimamectin itself for the chemical synthesis of moxictin. Synthesis, and lack of motivation to study the fermentation of neimamicin and its various components

Method used

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  • Binary product fermentation method and two kinds of fermentation products thereof
  • Binary product fermentation method and two kinds of fermentation products thereof
  • Binary product fermentation method and two kinds of fermentation products thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 The preparation of the composition containing neimamectin

[0038] Among the many neimamicin-producing bacteria, after a lot of experiments, we found Streptomyces NEM32G0 strain (available from Nuonong (Beijing) International Biotechnology Co., Ltd.), which cooperates with the fermentation and purification methods we have explored. Only through simple and low-cost preparation steps, without extraction and frequent use of chromatographic columns, etc., the neimamicin with a purity of more than 90% can be obtained, and more (about 1 / 3 of the amount of neimamicin) can be obtained at the same time ) impurities, the two can be used alone or in combination to prevent and control plant diseases and insect pests. Concrete preparation process is as follows:

[0039] 1. Activation of strains

[0040] The commercially available frozen Streptomyces NEM32G0 strain was inoculated into 1mL seed medium (recipe: glucose 20g, sucrose 10g, soybean powder 22g, yeast powder 5...

Embodiment 2

[0049] Example 2 The primary control effect of neimamectin and its fermentation impurities

[0050] We use the neimamectin prepared according to Example 1 (purity is more than 90%) (original drug A), impurity A (purity is more than 55%) (original drug Cl) and the impurity A purified with Sephadex G-15 (purity is more than 90%) (original drug C2) and impurity B (purity is more than 90%) (original drug D) purified with SephadexG-15, observe their ability to prevent and control mites.

[0051] Specifically, the original drug A was dissolved with an appropriate amount of methanol and diluted with water to different dilutions calculated as nemamycin, and the original drugs C1 and C2 were respectively dissolved with an appropriate amount of methanol and diluted with water to different dilutions based on impurity A. , Dissolve the original drug D with an appropriate amount of methanol and dilute it with water to different dilutions based on impurity D. The mites were inoculated on t...

Embodiment 3

[0054] The compound control effect of embodiment 3 neimamectin and its fermentation impurity

[0055] Preliminary tests have prompted us very unexpectedly that the neimamectin with a purity of more than 90% and the impurity A with a purity of more than 55% can be easily prepared according to the method of Example 1, both of which have a good effect on preventing and controlling mites. Because the extraction of impurity B is more difficult, and the control effect is worse than the former two, we compounded neimamectin and impurity A into the following groups of original drugs for testing:

[0056] Original drug A: naimamectin;

[0057] Original drug B: neimamectin + impurity A, the content ratio is 9:1;

[0058] Original drug C: neimamectin + impurity A, the content ratio is 5:5;

[0059] Original drug D: neimamectin + impurity A, the content ratio is 1:9;

[0060] Original drug E: impurity A

[0061] Each of the above equimolar total amounts (in terms of neimamectin+impuri...

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Abstract

The invention discloses a binary product fermentation method and two kinds of fermentation products thereof. The fermentation method for a composition containing Nemadectin comprises the steps of culture activation, cultivation enlarging, fermental cultivation and organic solvent purification, and recrystallization, chromatography and other complex steps are not needed. The invention further provides a composition and a pesticide preparation prepared through the fermentation method and the plant disease and pest preventing application.

Description

technical field [0001] The invention belongs to the field of fermentation and pesticides, in particular, the invention relates to a fermentation method and its binary product—fermented nemamycin and its fermentation impurities, which can be used to prevent and control plant diseases and insect pests. Background technique [0002] Nemadectin, also known as Nimoctin, is usually produced by Streptomyces fermentation. Naimamectin itself is mainly used as a synthetic intermediate of moxidectin, and is rarely used directly; and moxidectin is mainly used as a veterinary antibiotic for the treatment of parasitic diseases such as nematodes, ticks, and fleas in livestock. [0003] For example, Chinese Patent Application No. 201110242857 discloses a method for fermentatively producing nimoctine, but its research only involves the method itself for fermentatively producing nemoctin in a fermentation broth. [0004] Chinese patent application No. 201210414027 discloses a method for puri...

Claims

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Application Information

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IPC IPC(8): C12P17/18A01N63/02A01N43/90A01P7/02A01P7/04A01P5/00
Inventor 杨玉淮郭正
Owner 河北奥思德环保科技有限公司
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