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Stable fermentation method and doramectin composition thereof

A kind of doramectin and fermentation method technology, which is applied in the fields of fermentation and pesticides, and achieves the effect of being convenient for popularization and application

Active Publication Date: 2015-11-25
河北奥思德环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commercially available doramectins have a purity of more than 95%. Because they are mostly used in veterinary medicine, the purity is high, usually reaching more than 98%. No low-purity (for example, below 90%) doramectin is provided.

Method used

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  • Stable fermentation method and doramectin composition thereof
  • Stable fermentation method and doramectin composition thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of the composition that embodiment 1 contains doramectin

[0033] Among the many doramectin-producing bacteria, after a lot of experiments, we found Streptomyces strain D126 (available from Shijiazhuang Bairui Biotechnology Co., Ltd.), which cooperates with the fermentation and purification methods we have explored. , low-cost preparation steps, no need for crystallization, chromatographic column and other means, can obtain the doramectin composition with a purity of about 80%, and the impurity components are less and the proportion is basically stable. Concrete preparation process is as follows:

[0034] 1. Activation of strains

[0035] Commercially available frozen Streptomyces strain D126 was inoculated into a test tube of 1 mL of seed medium (recipe: 15 g of glucose, 5 g of sucrose, 15 g of soybean powder, 5 g of yeast powder, pH 7.0, and distilled water to 1 L), Cultivate at 150 rpm and 30° C. for 16 hours to obtain a strain activation solution. ...

Embodiment 2

[0048] Example 2 The primary control effect of the composition containing doramectin

[0049] We used the composition (technical drug A) with a purity of 80% of doramectin prepared according to Example 1 and commercially available doramectin with a purity of 99% (available from Hubei Xinrunde Chemical Co., Ltd.) ( The original drug B), in Guilin, carried out the control test on the bitter melon in the blooming stage which was infested by Thrips anthropus.

[0050] Dissolve the original medicines A and B with a small amount of ethanol and dilute them with water to 10ppm in terms of doramectin. Spray the whole bitter gourd until the water on the flowers and leaves of the plants starts to drip. Experiment was carried out on 5 bitter gourd plants randomly selected; the control group was sprayed with the same amount of water. Before spraying, 1 day after spraying, and 3 days after spraying, the number of living thrips animus in 10 flowers in each group was randomly investigated, a...

Embodiment 3

[0053] Example 3 The control effect of each composition in the composition containing doramectin

[0054] Preliminary tests have reminded us very unexpectedly that the doramectin-containing composition prepared in Example 1 is not only convenient to prepare and has good stability in doramectin content, but also has components that are regarded as impurity components that cannot be underestimated. preventive effect. For this reason, we separated and collected doramectin (that is, the peak at about 25min on the HPLC chromatogram), impurity A (that is, the peak at about 17.5min on the HPLC chromatogram) and impurity B in the composition. (i.e. the peak at about 13.4min on the HPLC chromatogram) and impurity C (i.e. the peak at about 12.2min on the HPLC chromatogram), due to the low content of each impurity, it is compounded with doramectin into the following groups Original drug to test:

[0055] Original drug A: doramectin;

[0056] Original drug B: doramectin + impurity A, t...

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Abstract

The invention provides a fermentation method for a composite containing doramectin. The method includes the steps of culture activation, enlarging cultivation, fermentation cultivation and organic solvent purification, and the complex steps of recrystallization, chromatography and the like are omitted. In addition, the invention further provides the composite prepared through the fermentation method, a pesticide preparation, disease and pest resistance application, and the like.

Description

technical field [0001] The invention belongs to the fields of fermentation and pesticides, in particular, the invention relates to a stable fermentation method and its product—a doramectin-containing composition, which also includes doramectin fermentation impurities. In addition, the present invention also relates to the application of the above-mentioned composition and the fermentation impurities therein. Background technique [0002] Doramectin, also known as Doramectin or Dectomax, is currently used as a veterinary drug, mainly used to treat ectoparasitic diseases such as nematodes and blood lice in livestock. For example, Chinese Patent No. 200910273301 and No. 200910273302 respectively disclose a veterinary anti-parasitic sustained-release injection and sustained-release oral preparation, in which commercially available doramectin can be used as the active ingredient of the anthelmintic; As another example, Chinese Patent No. 201010264540 discloses a doramectin micro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/62A01N43/90A01P7/04A01P7/02A01P5/00C12R1/465
Inventor 杨玉淮郭正
Owner 河北奥思德环保科技有限公司
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