Multichannel microfluidic-solid phase extraction-mass spectrum combined device and preparation method

Abstract

The invention discloses a multichannel microfluidic-solid phase extraction-mass spectrum combined device and a preparation method. The combined device comprises a microfluidic chip and a mass spectrometer, wherein the microfluidic chip is provided with upper and lower layers of channels; the upper layer of channel is a cell culture channel; the two ends of the upper layer of channel are provided with a vertical hole and a horizontal hole respectively; the lower layer of channel is a chemical oxygen uptake channel; the two ends of the lower layer of channel are provided with a plurality of groups of vertical holes respectively, and the upper and lower layers of channels are connected through a layer of PDMS (Polydimethylsiloxane) membrane; C18 spherical particles are filled on one side of the upper layer of channel to form a filling column; the horizontal hole of the upper layer of channel is connected with the mass spectrometer. The physiological behaviors of cells under different oxygen environments can be simultaneously observed, and control experiment, multi-concentration gradient parallel stimulation and multi-region detection can be realized at one time. Through separation and enrichment of the solid-phase extraction chip, mass spectrum real-time detection is realized, cell metabolites can be more intuitively and more accurately detected, and a theoretical basis is provided for research of tumor cell hypoxia.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a multi-channel microfluidic-solid phase extraction-mass spectrometry device and a preparation method. Background technique [0002] In recent years, microfluidic chip technology has continuously gained new applications and attempts in cell research. Since the biochemical reactions in cells are usually ultra-trace and millisecond level, high selectivity, high sensitivity, fast response and ultra-small volume of detection are required. In the initial microfluidic chip-cell research, fluorescence and electrochemical detection are often used for the detection of known or specific one or several target molecules, which can be focused to a fluorescent excitation spot or microelectrode with a diameter of tens of microns. The chemical electrodes can be well coupled with the micro-detection area on the chip to achieve high-sensitivity detection. However, the chemical composition...

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Problems solved by technology

Then the growth state of the cells is not consistent, which leads to different metabolic rates in the chip, and the cell density is prone to large errors. The repeatability of this method is poor.

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