Scale production method of MS basic solid culture substrate
A production method and solid culture technology, applied in the field of plant biology, can solve the problems of influence, different colors, manufacturers, inconsistent quality, etc.
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[0012] The present invention will be further described below in conjunction with specific embodiments.
[0013] In this embodiment, a large-scale production method of MS basic solid culture substrate includes five steps of accurate calculation, accurate weighing, first mixing less and then more, sealing, and unsealing for use.
[0014] The specific operation of the above five steps is as follows:
[0015] ① Accurate calculation: expand the 18 elements contained in the basic solid base of MS by 1000 times, that is, 0.025g each of cobalt chloride hexahydrate and copper sulfate pentahydrate, 0.1g of thiamine hydrochloride, 0.25g of sodium molybdate dihydrate, and 0.5g pyridoxine hydrochloride, 0.83g potassium iodide, 2g glycine, 6.2g boric acid, 8.6g zinc sulfate heptahydrate, 22.3g manganese sulfate tetrahydrate, 36.5g sodium iron edetate, 100g inositol, Potassium dihydrogen phosphate 170g, magnesium sulfate heptahydrate 370g, calcium chloride dihydrate 440g, ammonium nitrate 1...
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