Preparation method of biological preparation for preventing and controlling vegetable nematode disease

A biological preparation, nematode technology, applied in the fields of botanical equipment and methods, pesticides, biocides, etc., can solve the problems of unstable field use effect, incomplete root-knot nematode control and other problems, achieve bright color, Reduce the demand for pesticides and labor costs, the effect is good

Inactive Publication Date: 2015-06-24
山东金必来生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently used biocontrol agents are only Nematode Bic and Abamectin
Abamectin is mostly used in the control of vegetable root-knot nematodes, and abamectin granules can be used for furrow application o

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] Example one

[0027] A preparation method of a biological preparation for preventing and treating vegetable nematode disease is prepared by first culturing Paecilomyces lilacinus through a culture medium, and adding chitin to mixed culture after the culturing is mature.

[0028] Wherein, the medium is first prepared as follows: take 5-6 parts of raw beef extract, 3-4 parts of peptone, 100-200 parts of molasses, 10-20 parts of phosphorous acid, and 10-20 parts of potassium hydroxide according to parts by weight; Put the above raw materials into a fermenter, add water to 1000 parts, adjust the PH value to 6.5-7.5, heat to 110-130°C for 20-28 hours, then lower the temperature to 30-34°C.

[0029] Then put the Paecilomyces lilacinus into a fermentor, and agitate and aerate for cultivation. The culture temperature of Paecilomyces lilacinus is 28-32°C, the pH value is 6.5-7.5, and the culture is cultured for 5-7 days. The culture solution is matured, and then the chitin and the cul...

Example Embodiment

[0030] Example two

[0031] A preparation method of a biological preparation for preventing and curing vegetable nematode disease is prepared by first cultivating Paecilomyces lilacinus through a culture medium, and then adding chitin for mixed culture after the culturing is mature.

[0032] Wherein, the medium is first prepared as follows: take 5 kg of raw beef extract, 3 kg of peptone, 100 kg of molasses, 10 kg of phosphorous acid, and 10 kg of potassium hydroxide; put the above raw materials into a fermentation tank and add water to 1000 kg, Adjust the pH to 7, heat to 120°C for 24 hours, then lower the temperature to 32°C.

[0033] After that, the Paecilomyces lilacinus was put into the fermentor, the Paecilomyces lilacinus was put into the fermentor, and agitated and aerated for culture. The culture temperature of the Paecilomyces lilacinus was 32° C., and the pH value was 7 After culturing for 7 days, the culture solution is matured, and then the chitin and the culture solutio...

Example Embodiment

[0034] Example three

[0035] A preparation method of a biological preparation for preventing and curing vegetable nematode disease is prepared by first cultivating Paecilomyces lilacinus through a culture medium, and then adding chitin for mixed culture after the culturing is mature.

[0036] Wherein, the medium is first prepared as follows: take 6 kg of raw beef extract, 4 kg of peptone, 200 kg of molasses, 20 kg of phosphorous acid, and 20 kg of potassium hydroxide; put the above raw materials into a fermentation tank and add water to 1000 kg, Adjust the pH to 7, heat to 130°C for 28 hours, and then lower the temperature to 30°C.

[0037] After that, the Paecilomyces lilacinus was put into the fermentor, the Paecilomyces lilacinus was put into the fermentor, and agitated and aerated for culture. The culture temperature of the Paecilomyces lilacinus was 30°C and the pH value was 7. , Cultivate for 5 days to mature the culture solution, then mix the chitin and the culture solution ...

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PUM

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Abstract

The invention discloses a preparation method of a biological preparation for preventing and controlling a vegetable nematode disease. The preparation method comprises the steps of firstly culturing paecilomyces lilacinus by virtue of a culture medium until mature, adding chitosan, and carrying out mixed culture, so as to obtain the biological preparation. According to the preparation method, paecilomyces lilacinus and chitosan are well combined together and do not need to be separately used, so that the use is convenient, the cost is low, the effect is good; furthermore, the prepared biological preparation for preventing and controlling the vegetable nematode disease can be used during soil preparation fertilization, growing period and flowering and fruit bearing periods, is stable in field use effect and has efficacies of enhancing fertility, increasing field, improving quality, saving labor, increasing benefit, reducing residual toxicity, improving soil quality and eliminating nematodes.

Description

technical field [0001] The invention relates to a biological agent, in particular to a preparation method of a biological agent for preventing and treating vegetable nematode disease. Background technique [0002] The root-knot nematode mainly damages the vegetable roots, and the lateral roots and fibrous roots of vegetables are the most vulnerable. Nodules or root knots of different sizes are produced on the affected roots. The root knots are white and soft at first, and then become light yellowish brown or dark brown. The surface is rough and sometimes cracked. When the root knot is cut open, small white pearly particles can be seen, which are female adults of root knot nematode. Vegetables grow slowly after being infected by root-knot nematodes, and the leaves are small and yellow. When the disease is severe, the plants are short, not firm or poorly firm. In high temperature and dry weather, the plants are easy to wilt or even die. Plant nematodes often occur locally in...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P7/04
Inventor 裴德修尚文燕裴晓婷王晨
Owner 山东金必来生物科技有限公司
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