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Preparation method of total flavonoids of clematis filamentosa Dunn and application of total flavonoids of clematis filamentosa Dunn to drug for treating myocardial ischemia

A technology of anti-myocardial ischemia and total flavonoids, which is applied in the direction of drug combination, cardiovascular system diseases, medical preparations containing active ingredients, etc., can solve the problems of unfavorable development of new drug and new dosage form, unfavorable drug efficacy, low content, etc. Achieve good anti-myocardial ischemia, improve blood rheology, high purity effect

Inactive Publication Date: 2015-06-24
广东省中药研究所
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Problems solved by technology

[0009] Ganmotong has good medicinal effects in the treatment of coronary heart disease, hypertension, etc., but the current research and clinical application of Ganmotong are all on its crude extract. The content in the extract does not exceed 1.0%, and the low content is not conducive to the full play of its medicinal effect, nor is it beneficial to the development of new drugs and new dosage forms.

Method used

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  • Preparation method of total flavonoids of clematis filamentosa Dunn and application of total flavonoids of clematis filamentosa Dunn to drug for treating myocardial ischemia
  • Preparation method of total flavonoids of clematis filamentosa Dunn and application of total flavonoids of clematis filamentosa Dunn to drug for treating myocardial ischemia
  • Preparation method of total flavonoids of clematis filamentosa Dunn and application of total flavonoids of clematis filamentosa Dunn to drug for treating myocardial ischemia

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Embodiment 1

[0026] Embodiment 1: A kind of adopting heating reflux extraction method, through macroporous resin-silica gel chromatography secondary separation, the method for preparing the total flavonoids of Amoyaceae, its steps are as follows:

[0027] Take 5kg of the aboveground part of Ganmotong, crush it into a coarse powder, add 18 times the amount of 70% ethanol, heat and reflux for extraction twice, extract for 2h and 1h respectively, filter, combine the filtrates of the two times, recover ethanol under reduced pressure, and concentrate to a specific gravity of 1.15. Obtain Ganotong extract; disperse the Ganktong extract evenly with 12 times the amount of water, place it for precipitation for 12 hours, filter, and concentrate the filtrate under reduced pressure to a specific gravity of 1.12 to obtain the Ganmutong alcohol-extracted water-sink liquid; D101 macroporous resin adsorption column on the liquid, wash the impurities with water, and then wash the impurities with 15% ethanol...

Embodiment 2

[0028] Embodiment 2: A method for preparing the total flavonoids of Glycyrrhiza radicis through macroporous resin-silica gel chromatography secondary separation using ultrasonic-reflux cooperative extraction method, the steps are as follows:

[0029] Take 5kg of the above ground part of Amoyaceae, crush it into a coarse powder, add 12 times the amount of 70% ethanol, add 1.0% sodium lauryl sulfate, and extract by ultrasonic heating and reflux for 45min, the ultrasonic power is 200W, filter, and the filtrate is decompressed to recover ethanol Concentrate to a specific gravity of 1.15 to obtain Ganmutong extract; disperse the Ganmutong extract evenly with 10 times the amount of water, place it for precipitation for 12 hours, filter, and concentrate the filtrate under reduced pressure to a specific gravity of 1.12 to obtain Ganmutong alcohol extraction water sink liquid; AB-8 macroporous resin adsorption column is used to extract the water sedimentation liquid of Tongalcohol, wash...

Embodiment 3

[0030] Embodiment 3: A kind of adopts enzymolysis-reflux extraction method, through macroporous resin-silica gel chromatography secondary separation, the method for preparing the total flavonoids of Amoyaceae, its steps are as follows:

[0031] Take 5kg of the above ground part of Ganmotong, crush it into a coarse powder, add 12 times the amount of 60% ethanol, add 2.0% cellulase, adjust the pH value to 5.5, heat at 60°C for reflux extraction twice, extract for 2h and 1h respectively, filter , the filtrate was decompressed to recover ethanol, concentrated to a specific gravity of 1.14 to obtain Ganmotong extract; the Ganmutong extract was uniformly dispersed with 10 times the amount of water, placed for 12 hours to precipitate, filtered, and the filtrate was concentrated under reduced pressure to a specific gravity of 1.13 to obtain Ganmutongol Extract water and sink liquid; put amoxicillin extract water and sink liquid on HPD400 macroporous resin adsorption column, wash the im...

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Abstract

The invention belongs to the field of traditional Chinese medicines and particularly relates to a preparation method of total flavonoids of clematis filamentosa Dunn and application of the total flavonoids of clematis filamentosa Dunn to a drug for treating myocardial ischemia. The main technical scheme is as follows: whole dried herbs on the overground part of clematis filamentosa Dunn serving as a traditional Chinese medicine are extracted, chromatographically separated and purified to prepare the total flavonoids of clematis filamentosa Dunn, wherein the purity of the total flavonoids of clematis filamentosa Dunn is more than 85%. In-vitro and in-vivo pharmacological experiments prove that the prepared total flavonoids of clematis filamentosa Dunn can be used for treating myocardial ischemia, reducing blood viscosity and reducing platelet aggregation, and have a certain protecting effect for ischemic myocardium cells and a remarkable myocardial ischemia treating effect. The high-purity total flavonoids of clematis filamentosa Dunn are prepared from clematis filamentosa Dunn medicinal materials serving as raw materials through macroporous resin and silica gel chromatographic coupled separation, the total flavonoids of clematis filamentosa Dunn can be prepared into a preparation such as a tablet, a capsule, a dropping pill and a powder injection by adding auxiliary materials, and the preparation can be applied to treatment of myocardial ischemia diseases such as latent coronary heart disease, angina, myocardial infarction and ischemic cardiomyopathy. The preparation method disclosed by the invention is simple and stable in process and is easily produced in batches.

Description

Technical field: [0001] The invention belongs to the field of traditional Chinese medicine, and relates to an anti-myocardial ischemia drug, which is used for the prevention and treatment of myocardial ischemic diseases such as occult coronary heart disease, angina pectoris, myocardial infarction, ischemic cardiomyopathy, etc. The preparation method of total flavonoids and its application in anti-myocardial ischemia drugs. Background technique: [0002] Coronary atherosclerotic heart disease (CAD) is mostly caused by the imbalance between the reduction of coronary blood flow and myocardial demand caused by coronary artery disease, that is, myocardial ischemia, resulting in myocardial damage. The clinical manifestations are angina pectoris, myocardial Infarction (myocardial infarction, MI), arrhythmia, sudden death or heart failure, the resulting heart disease is often called "coronary heart disease". Coronary heart disease not only has a high incidence rate, but also leads ...

Claims

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Application Information

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IPC IPC(8): A61K36/716A61P9/00A61P9/10A61P7/02
Inventor 聂阳张现涛姚万龙何盛江
Owner 广东省中药研究所
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