1results about How to "Increase surface charge" patented technology

The invention discloses a horseradish peroxidase mutant with improved thermal stability and construction and application of genetically engineered bacteria of the horseradish peroxidase mutant, and belongs to the technical field of biology. The horse radish peroxidase mutant capable of efficiently catalyzing oxidation reaction of various organic and inorganic substrates is constructed, compared with wild type HRP, the half-life period of the mutant at the temperature of 50 DEG C is prolonged, and the half-life period of double mutants can be prolonged to 4 times that of the wild type HRP. The horse radish peroxidase variant disclosed by the invention has a wide application prospect, particularly shows excellent catalytic efficiency and stability under harsh reaction conditions, and provides a better enzyme tool for the fields of industry and biomedicine.