The invention discloses a preparation method of bamboo fiber paper cups. The invention relates to a bamboo fiber paper cup and a production process thereof. The bamboo fiber paper cup mainly structurally comprises a hollow fiber cup body, and is characterized in that the cup body comprises an outer layer, an inner layer, a hollow layer arranged between the outer layer and the inner layer and a coating layer above the inner layer. The outer layer and the inner layer are prepared from the following components in parts by weight: bleached bamboo fibers, dahurian larch fibers, modified starch, chitosan, talc powder, a dispersant, guar gum, glyceride hydrolase and a wet-strength agent. The hollow layer is prepared from the following components in parts by weight: polypropylene hollow short fiber pulp, dahurian larch fibers, chitosan, talc powder and a dispersant. According to the above settings, the outer layer and the inner layer are produced by employing bamboo fibers. The bamboo fiber pulp not only is environment-friendly, but also has the characteristics of being excellent in antibacterial property, bacteriostatic and free of peculiar smells. The hollow fiber layer has the functionsof being light in weight, heat insulative and warm-keeping. The bamboo fiber paper cup serving as a hot drink paper cup and a cold drink paper cup simultaneously is relatively safe.