The invention belongs to the technical field of hypsizygus marmoreus cultivation, and particularly relates to a hypsizygus marmoreus cultivation large-scale high-yield technology which comprises the following steps: 1, selecting seeds; 2, preparing a culture medium, and controlling the pH value of the culture medium; 3, mixing the optimized strain into the culture medium, and putting the culture medium into a culture bag; 4, the culture bags are stacked in a greenhouse, and the temperature, humidity and carbon dioxide concentration in the greenhouse are controlled; 5, waiting for spawn running, ventilating for 3-4 times per day and 25-30 minutes per time, and waiting for 30-35 days until the culture bag is full of hyphae; 6, artificial bud pressing; step 7, fruiting; according to the method, high-quality strains are selected in advance, so that products with poor quality during fruiting are reduced, and selling is facilitated; meanwhile, in the application document, a special culture medium preparation method and a raw material ratio are adopted, the hypsizygus marmoreus spawn running time is shortened, and meanwhile, artificial bud pressing is adopted, so that the production efficiency of hypsizygus marmoreus is improved.