A kind of Rhodococcus and its application in degrading lignin

A technology of lignin and rhodococcus, applied in the field of microorganisms, can solve the problems of difficult degradation, environmental pollution, high energy consumption of physical methods, etc., and achieve the effect of low cost and no secondary pollution

Active Publication Date: 2017-10-20
JIANGSU UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The combination of lignin with cellulose and hemicellulose is very stable and not easy to degrade. It is one of the key bottlenecks in the utilization of biomass energy. At the same time, lignin is also a by-product of the wood hydrolysis industry and paper industry and a relatively difficult to degrade in municipal solid waste. The use of chemical reagents to treat these wastes will cause serious environmental pollution
[0003] Although there have been decades of research on lignin degradation methods, there are still many problems, such as chemical methods cannot solve the pollution problem, physical methods have the challenge of high energy consumption, and biodegradation due to its low cost, the reaction The advantages of mild conditions and no secondary pollution are widely used in the degradation of various harmful organic substances

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Rhodococcus ( Rhodococcus sp.) L17 was isolated and purified from sea mud in the South China Sea.

[0022] The separation and purification method is as follows: the acclimatization medium used is inorganic salt medium, and the composition is (g / L): 6.2gNa 2 HPO 4 , 3.0g KH 2 PO 4 , 0.5g NaCl, 1.0g NH 4 Cl, pH 7.0. First, add 3g of sludge to 100mL of inorganic salt medium containing 20mg / L lignin, and after acclimatization at 37°C for 60 days, insert 10% inoculum volume of lignin into inorganic salt medium containing 40mg / L of lignin, and then acclimate for one week, followed by By analogy, gradually increase the concentration of lignin for domestication, and the concentrations of lignin are 40mg / L, 60mg / L, and 80mg / L respectively.

[0023] After the acclimatization, spread the acclimatization solution on the agar plate (beef extract 3g / L, peptone 10g / L, sodium chloride 5g / L, agar powder 20g / L, pH7.0) and culture at 37°C for 48h, and select a single colony on Bee...

Embodiment 2

[0034] Rhodococcus ( Rhodococcus sp.) L17 single colony was inoculated in 3mL beef extract peptone medium, cultured for 18h (OD 600 0.45) with an inoculum volume of 20% by volume, it was inserted into an inorganic salt medium with a lignin concentration of 20 mg / L and a pH of 8.0, reacted at 37°C and a shaker at 150 rpm for 96 hours, and measured the degradation by UV spectrophotometry Rate. The mensuration of degradation rate is the same as embodiment 1, and detection result is that degradation rate is 78%.

Embodiment 3

[0036] Rhodococcus ( Rhodococcus sp. .) A single colony of L17 was inoculated in 3 mL of beef extract peptone medium, and the culture cultured for 18 hours was inserted into an inorganic salt medium with a lignin concentration of 60 mg / L and a pH of 7.0 at an inoculation volume of 40% by volume. ℃, 100rpm shaker reaction for 108h, the degradation rate was measured by ultraviolet spectrophotometry, the measurement method was the same as in Example 1, and the test result was that the degradation rate was 82%.

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Abstract

The invention relates to a Rhodococcus and its application in degrading lignin, belonging to the technical field of microorganisms; the strain is obtained by separating and purifying the sea mud of the South China Sea, and is a variant of the genus Rhodococcus named (Rhodococcussp. ) L17, which was identified as Gram-positive, non-conidia-forming, aerobic, catalase-positive, and arylsulfatase-negative; and its 16S rDNA sequence has been submitted to the NCBI database with accession number KM215139 , and was preserved on March 20, 2014 in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is CGMCC8943; it has been verified by experiments that the strain has the ability to degrade lignin, and the degradation rate can reach 90%‑100% , which can be applied in the field of environmental protection, especially in the biodegradation of lignin.

Description

technical field [0001] The invention relates to a rhodococcus and its application in degrading lignin, belonging to the technical field of microorganisms. Background technique [0002] Lignin is one of the three most abundant biomass in nature, accounting for 15%-40% of the dry weight of terrestrial plants. Its structure is an amorphous three-dimensional spatial structure composed of phenylpropane unit structure through ether bond and carbon-carbon bond. Aromatic compounds, together with cellulose and hemicellulose, are the main components of the plant skeleton. The combination of lignin with cellulose and hemicellulose is very stable and not easy to degrade. It is one of the key bottlenecks in the utilization of biomass energy. At the same time, lignin is also a by-product of the wood hydrolysis industry and paper industry and a relatively difficult to degrade in municipal solid waste. The use of chemical reagents to treat these wastes will cause serious environmental poll...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/20C12P7/64C12R1/01
CPCC12N1/205C12R2001/01
Inventor朱道辰孙建中谢长校李成林张佩佩
OwnerJIANGSU UNIV