A method for improving seedless grape hybrid embryo rescue into seedlings

A seedless grape and hybrid embryo technology, applied in the fields of botanical equipment and methods, plant genetic improvement, application, etc., can solve the difficulty of increasing the technical system of seedless grape embryo rescue, different optimal periods of embryo abortion, and embryo development. It can reduce the risk of single plant loss, promote embryo germination, and achieve the effect of high development rate.

Active Publication Date: 2017-05-31
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of embryo rescue, the genotype of the variety, the time of sampling and inoculation, the type and phase of the medium, plant growth regulators, carbon sources, culture environment, transplanting methods, etc. will all have an impact on the germination rate and seedling rate of embryo rescue. , and the interaction of various factors, and the differences between the north and the south make the southern grape mature early and the best period of embryo abortion is different, which increases the difficulty of constructing the seedless grape embryo rescue technology system, resulting in the seedless grape being the female parent. The embryo development rate in the fruit after hybridization is low, and the lack of embryo rescue has become a bottleneck restricting the breeding efficiency of seedless grapes in the south. How to increase the seedling rate of seedless grape embryo rescue breeding and expand the population of embryo rescue plants has become a key issue for grape breeders. Striving Direction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for improving seedless grape hybrid embryo rescue into seedlings, comprising the following steps,

[0018] A. Topping and girdling: the seedless grape female parent grows slightly to the 6-leaf stage, leaves 1 leaf on the inflorescence for topping treatment, and performs a second topping treatment on the top auxiliary shoots 7 days before flowering, leaving 1 leaf for the remaining auxiliary shoots Pinching, before artificial castration at the initial flowering stage, pinching of the top and auxiliary shoots and girdling of fruiting branches;

[0019] B. Sampling after hybridization and inoculation of ovules after low temperature treatment: artificial detassellation at the initial flowering stage, picking young fruits at the optimal fruiting stage of hybrid seed abortion after pollination, and treating young fruits at 4°C after retrieval for 3 Day, the ovules were stripped and inoculated, the B5 liquid medium added with the first exogenous supplement was select...

Embodiment 2

[0026] A method for improving seedless grape hybrid embryo rescue into seedlings, comprising the following steps,

[0027] A. Topping and girdling: the seedless grape female parent grows slightly to the 6-leaf stage, leaves 1 leaf on the inflorescence for topping treatment, and performs a second topping treatment on the top auxiliary shoots 7-10 days before flowering, and the remaining auxiliary shoots are kept 1 leaf pinching, before the artificial castration at the initial flowering stage, the pinching of the top and auxiliary shoots and the girdling of the fruiting branches are carried out;

[0028] B. Sampling after hybridization and inoculation of ovules after low temperature treatment: artificial detassellation at the initial flowering stage, picking young fruits at the best fruiting stage when the hybrid seeds are aborted after pollination, and treating them at 6°C for 3 Day, the ovules were stripped and inoculated, the B5 liquid medium added with the first exogenous su...

Embodiment 3

[0036] A method for improving seedless grape hybrid embryo rescue into seedlings, comprising the following steps,

[0037] A. Topping and girdling: the seedless grape female parent grows slightly to the 6-leaf stage, leaves 1 leaf on the inflorescence for topping treatment, and performs a second topping treatment on the top auxiliary shoots 7-10 days before flowering, and the remaining auxiliary shoots are kept 1 leaf pinching, before the artificial castration at the initial flowering stage, the pinching of the top and auxiliary shoots and the girdling of the fruiting branches are carried out;

[0038]B. Sampling after hybridization and inoculation of ovules after low temperature treatment: artificial detassellation at the initial flowering stage, picking young fruits at the best fruiting stage when the hybrid seeds are aborted after pollination, and treating them at a low temperature of 5°C after the young fruits are retrieved, 3 On the first day, the ovules were stripped and...

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PUM

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Abstract

The invention belongs to the technical field of plant breeding and discloses a method for improving the seedling recovery of seedless grape hybrid embryos. Before the seedless grape hybrid embryos are aborted, the physical measures of pinching and girdling are used to promote the internalization of ovules and embryos. Development, determine the appropriate time for embryo abortion rescue sampling of two southern seedless grape hybrid combinations, determine the medium supplements for ovule culture and embryo germination, the inoculation and subculture of test-tube plantlets with double-bud stems and the addition of subculture medium things etc. The seedless grape hybrid embryo development rate obtained by applying the present invention is high, the embryo rescued seedlings are neat and strong, the embryo development rate has reached 41.7%, the seedling rate has reached 18.3%, and the seedling rate of subculture has reached 92%. The seedling rate reached 81.8%, and the survival rate of transplanted test-tube seedlings reached 97%, which greatly increased the base number of hybrid offspring embryos to save seedlings, so that more seedless hybrid offspring plant populations could be obtained, and the seedless grape hybridization in the south was improved. Embryo rescue seedling rate, the method is highly operable and easy to master.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for improving seedless grape hybrid embryo rescue into seedlings. Background technique [0002] Seedless grapes are popular among consumers due to their convenience, and occupy an important position in the production of table grapes in the world. More than 80% of the table grapes in the United States are seedless varieties. However, in terms of grape breeding, since seedless grapes have no seeds, it is impossible to obtain hybrid offspring plants as female parents. Very low, and the breeding cycle is long. In the 1990s, the development of embryo rescue technology brought a new approach to seedless grape breeding, making it possible for seedless grapes to be used as female parents for hybrid breeding, which not only greatly increased the probability of seedless offspring, but also greatly shortened the breeding time. Cycle, to a certain extent, solves the difficu...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): A01H1/02
Inventor程建徽郑婷吴江魏灵珠
OwnerZHEJIANG ACADEMY OF AGRICULTURE SCIENCES