Asarum total polysaccharides extract with cough relieving activity as well as extraction method and application thereof

An extraction method and technology of total polysaccharides, applied in the direction of organic active ingredients, medical preparations containing active ingredients, drug combinations, etc., to achieve good reproducibility, significant antitussive effect, and good drug safety effects

Active Publication Date: 2015-07-29
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT) +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, there are few research reports on the non-volatile components of Asarum. Bell amide Ⅰ, kaempferol-3-O-glucoside, etc. (Cai Shaoqing. Research on the non-volatile chemical components of Asarum. Beijing Medical University Journal. 1996,28(3):228-230; Shuai Lv. Liaoshixin Chemical constituents of the underground part (Ⅱ). Journal of Shenyang Pharmaceutical University. 2010,27(9):707-710; Wei Qingchun. Isolation and identification of chemical constituents of Hancheng Asarum. Shi Zhen Guo Yi Guo Yao. 2010,21(3) :676-677)
However, there is no research report on the antitussive activity of the non-volatile components of Asarum asarum total polysaccharides

Method used

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  • Asarum total polysaccharides extract with cough relieving activity as well as extraction method and application thereof
  • Asarum total polysaccharides extract with cough relieving activity as well as extraction method and application thereof
  • Asarum total polysaccharides extract with cough relieving activity as well as extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation method of Asarum total polysaccharide extract described in the present embodiment comprises the following steps:

[0051] 1 kg of dried Asarum root and / or rhizome, crushed into coarse powder, passed through a 80-mesh sieve, extracted with water 10 times the weight of the material, heated and refluxed for 5 hours, the extract was filtered through 6 layers of gauze, concentrated under reduced pressure Dried to obtain the total extract of Herba Asarum;

[0052] Heat and dissolve the total extract of Herba Asarum with water to 1L, add 9 times the volume of absolute ethanol until the volume percentage concentration of alcohol is 90%, stir while adding, after sufficient precipitation, let it stand overnight in the refrigerator at 4°C, and pour the supernatant solution, the precipitate was filtered under reduced pressure, washed twice with an equal amount of 90% by volume ethanol aqueous solution, and dried conventionally to obtain the total polysaccharide extr...

Embodiment 2

[0054] The preparation method of Asarum total polysaccharide extract described in the present embodiment comprises the following steps:

[0055] 1 kg of dried Asarum root and / or rhizome, crushed into coarse powder, passed through an 80-mesh sieve, extracted with water 10 times the weight of the material, heated and refluxed for 5 hours, the extract was filtered through 6 layers of gauze, and concentrated under reduced pressure Dried to obtain the total extract of Herba Asarum;

[0056] Heat and dissolve the total extract of Herba Asarum with water to 1L, add 15 times the volume of absolute ethanol until the volume percentage concentration of alcohol is 94%, stir while adding, after sufficient precipitation, let it stand in the refrigerator at 4°C overnight, and pour the supernatant solution, the precipitate was filtered under reduced pressure, washed 3 times with an equal amount of absolute ethanol, and conventionally dried to obtain the total polysaccharide extract of Asarum ...

Embodiment 3

[0058] Acute Toxicity Test of Asarum Total Polysaccharide Extract in Mice Orally

[0059] 1 experimental animal

[0060] SPF-grade NIH mice, half male and half female, weighing 18-22 g, were provided by the Guangdong Provincial Medical Experimental Animal Center, animal qualification certificate number: SCXK (Guangdong) 2008-0002.

[0061] 2 Experimental methods

[0062]Referring to "Pharmacological Experimental Methodology" and "Chinese Medicine Pharmacological Experimental Methodology", the formal Bliss method was used to investigate the acute toxicity of Asarum total polysaccharide extract administered orally to mice. Firstly, experiment with the maximum concentration, maximum dosage, and maximum gavage volume. Before the test, fasting can not help water 12h test by 10.0g / kg Asarum total polysaccharide extract (obtained according to the preparation method described in Example 1) orally (equivalent to crude drug amount 24.4g / kg, equivalent to 12 times of clinical dosage )...

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Abstract

The invention discloses a Manchurian wildginger total polysaccharide extract with cough-relieving activity and a preparation method and application thereof, belonging to the technical field of medicines. The Manchurian wildginger total polysaccharide extract is prepared by subjecting the root and / or tuber of Manchurian wildginger to extraction with a polar solvent, carrying out ethanol precipitation and then carrying out drying under reduced pressure, wherein the yield and the content of the total polysaccharide are 15 wt% and 60 to 80 wt%, respectively. Through a great number of pharmacodynamic tests, it is proved for the first time that the Manchurian wildginger total polysaccharide extract has a substantial cough inhibiting effect which is close to a treatment effect of codeine and shows a better effect on prolonging the incubation period of cough compared with codeine, and it is found out for the first time that the Manchurian wildginger total polysaccharide extract is capable of reducing sensitivity of cough and inhibiting airway inflammations, has good prevention and treatment effects on a variety of cough and can be used for preparation of drugs used for treating diseases related to cough. The Manchurian wildginger total polysaccharide extract has the advantages of definite activity, usage of original plants as experimental materials and low cost.

Description

technical field [0001] The invention belongs to the technical field of medicines, and more specifically relates to an extract of asarum total polysaccharides with antitussive activity, an extraction method and application thereof. Background technique [0002] Due to many factors such as work pressure and air pollution, the incidence of human respiratory diseases remains high, and cough is a common and frequently-occurring disease of the respiratory system, and it is a common complaint in respiratory clinics. Cough itself is a defensive reflex activity produced when the respiratory system is stimulated. It is a beneficial action and is sometimes seen in healthy people. In general, mild and infrequent coughs can be relieved naturally without the use of cough medicine. In addition, the impact of cough on human health is also dialectical, and it is also a pathological response. Long-term frequent or too intense cough will not only increase the suffering of patients, affect res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P11/14
CPCA61K36/268A61K2236/00A61K31/715A61P11/14A61K2236/30
Inventor 赖克方刘晓东钟伯年钟山聂怡初
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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