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Detection method of tetrodotoxin

A technology of tetrodotoxin and detection method, which is applied in the biological field and can solve the problems of poor repeatability and inaccurate quantification.

Inactive Publication Date: 2015-08-05
QINZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mouse method is the most commonly used and easiest method. Liao Yongyan et al. also used the mouse method to detect tetrodotoxin in Chinese horseshoe crabs and horseshoe crabs in 2000, but this method has the disadvantages of inaccurate quantification and poor repeatability.

Method used

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  • Detection method of tetrodotoxin
  • Detection method of tetrodotoxin
  • Detection method of tetrodotoxin

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0032] The present invention will be further described below with embodiment, but the present invention is not limited to these embodiments.

[0033] Materials and Methods

[0034] The adult horseshoe crabs of China were purchased from Zhanjiang Seafood Wholesale Market; the adult horseshoe crabs were collected from Anhai District, East China Sea Island, Zhanjiang.

[0035] instrument

[0036] Japan Shimadzu EL-120HA electronic balance (sensitivity 0.01g), Japan Shimadzu AY120 electronic balance (sensitivity 0.0001g), Tianjin Jinteng Experimental Equipment Co., Ltd. GM-0.33Ⅱ vacuum diaphragm suction filter, Keda Innovation Co., Ltd. The company's KDC-160H highly refrigerated centrifuge. Japan Shimadzu LC-20AT high performance liquid chromatography, Japan Shimadzu SPD-20A visible-ultraviolet detector, SunFire TM C18 chromatographic column (4.6mm×150mm), Italy Hanna HI8424 pH meter, Beijing Puxi General Instrument Co., Ltd. TU1901 UV-Vis spectrophotometer.

[0037] Reagent ...

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Abstract

The invention relates to a detection method of tetrodotoxin in tachypleus tridentatus and carcinoscorpius rotundicauda, which includes the following steps: (1) preparing a standard stock solution; (2) pre-treating a sample; and (3) performing liquid chromatographic test. In the invention, a high performance liquid chromatographic method is used for detecting the tetrodotoxin in tachypleus tridentatus and carcinoscorpius rotundicauda for determining whether toxin in horseshoe crabs belongs to tetrodotoxin or not, thereby knowing what kinds of the horseshoe crab causing a person to be suffered from horseshoe crab poisoning and what the content is, thereby providing reference to prevention and treatment of horseshoe crab poisoning.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for detecting tetrodotoxin in Chinese horseshoe crabs and horseshoe crabs. Background technique [0002] Chinese horseshoe crabs are mainly distributed in Japan's Seto Inland Sea and the north coast of Kyushu Island, Vietnam, islands in the West Philippines, Sumatra and Java Island, North Borneo in Malaysia, and the east coast of Kalimantan. In China, they are mainly distributed in the Yangtze River. The coastal waters of Zhejiang, Fujian, Guangdong, Guangxi, Hainan, and Taiwan in the south are mostly in Fujian, Guangdong, Guangxi, and Hainan. Horseshoe crabs are distributed in Hong Kong, Beibu Gulf, Leizhou Bay, north of the north coast of Java Island, Indonesia, west of southern Philippines and east of northeastern India at the mouth of the Ganges River. In my country, horseshoe crabs are mainly distributed in the Beibu Gulf, the west coast of Leizhou Peninsula, and the wes...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 廖永岩
Owner QINZHOU UNIV