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A method for extracting anti-ultraviolet radiation active substances from seaweed

A technology of radiation activity and seaweed, which is applied in pharmaceutical formulations, skin care preparations, cosmetics, etc., to achieve product safety, low production costs, and shorten the extraction time

Active Publication Date: 2017-08-22
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the preparation technology of the anti-ultraviolet radiation active substances of seaweed, and there is still a lot of room for development

Method used

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  • A method for extracting anti-ultraviolet radiation active substances from seaweed
  • A method for extracting anti-ultraviolet radiation active substances from seaweed
  • A method for extracting anti-ultraviolet radiation active substances from seaweed

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The method for extracting the anti-ultraviolet radiation active substance from the seaweed provided by the present embodiment comprises the following steps:

[0040] (1) Pretreatment: Wash the freshly collected seaweed to remove the sediment, drain the water, and then dry it in a freeze dryer at -60°C for 24 hours. Take the freeze-dried seaweed and grind it intermittently in a tissue grinder or use a high-speed The flux tissue grinder is crushed, and the crushed pineapple algae powder is passed through a 60-mesh mesh sieve, and the 60-mesh algae powder is collected and stored in a sealed bag;

[0041] (2) Ultrasonic method extraction: first the applicant of the present invention has carried out optimum extraction process condition research, has studied the influence of methanol concentration on extraction rate, the selection of solid-liquid ratio, the selection of ultrasonic treatment time, the selection of ultrasonic treatment temperature, The influence of ultrasonic p...

Embodiment 2

[0055] The method for extracting the anti-ultraviolet radiation active substance from the seaweed provided by the present embodiment comprises the following steps:

[0056] (1) Pretreatment: Wash the freshly collected seaweed to remove the sediment, drain the water, and then dry it in a freeze dryer at -55°C for 40 hours. Take the freeze-dried seaweed and grind it intermittently in a tissue grinder or use a high-speed The flux tissue grinder is crushed, and the crushed pineapple algae powder is passed through a 60-mesh mesh sieve, and the 60-mesh algae powder is collected and stored in a sealed bag;

[0057] (2) Ultrasonic extraction: put the seaweed powder in the extraction container, add methanol aqueous solution with a concentration of 5%, the ratio of solid to liquid between the two is 1:55, extract in an ultrasonic instrument, and control the extraction temperature The temperature is 35°C, the extraction time is 70min, the ultrasonic frequency is 35kHz, and the ultrasonic...

Embodiment 3

[0063] The method for extracting the anti-ultraviolet radiation active substance from the seaweed provided by the present embodiment comprises the following steps:

[0064] (1) Pretreatment: Wash the freshly collected seaweed to remove the sediment, drain the water, and then dry it in a freeze dryer at -58°C for 35 hours. Take the freeze-dried seaweed and grind it intermittently in a tissue grinder or use a high-speed The flux tissue grinder is crushed, and the crushed pineapple algae powder is passed through a 60-mesh mesh sieve, and the 60-mesh algae powder is collected and stored in a sealed bag;

[0065] (2) Ultrasonic extraction: put the seaweed powder in the extraction container, add methanol solution with a concentration of 15%, the ratio of solid to liquid is 1:65, and extract in an ultrasonic instrument, controlling the extraction temperature to 50°C , the extraction time is 85min, the frequency of ultrasonic wave is 35kHz, the intensity of ultrasonic wave is 280W, th...

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Abstract

The invention discloses a method for extracting anti-ultraviolet radiation active substances from seaweed, which comprises the following steps: (1) pretreatment; (2) ultrasonic extraction; (3) freezing and alcohol precipitation; (4) centrifugal removal of impurities; (5) Decolorization to obtain a white anti-ultraviolet radiation active substance. The method adopts an integrated technology combining low-concentration methanol, ultrasonic technology extraction and solid-phase small column separation and purification technology, and maximizes the extraction of the Active substances, the anti-ultraviolet radiation active substances of the seaweed extracted by this method have obvious ultraviolet light absorbing ability in the UVB (280~320nm) segment and 320~360nm (UVA), which is very suitable for cosmetics and has a great effect on daily sun protection. Obvious effect.

Description

technical field [0001] The invention belongs to the technical field of seaweed, and in particular relates to a method for extracting anti-ultraviolet radiation active substances from seaweed. Background technique [0002] Sea radish, also known as red cabbage, cow hair vegetable, gum vegetable, etc. Rhodophyta Cladophyceae Cladophyceae genus, annual red algae. The algal body is purple-red, yellowish-brown to brown, soft leathery, tough after drying, 4-10cm high, up to 15cm, clustered, short main branch, cylindrical or sub-cylindrical, about 4mm wide, irregular dichotomous branches , often constricted at the branch. Seaweed likes to grow on rocky reefs outside the sea that are impacted by waves and have high salinity. The vertical distribution can reach about 2 meters, and it likes sunshine. my country's coastal areas are distributed in Liaoning, Hebei, Shandong, Jiangsu, Zhejiang, Fujian, Guangdong, Taiwan and other coastal areas, and the southeast coast is widely distrib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9717A61Q17/04
Inventor 吴燕燕李来好杨贤庆张婉王锦旭陈胜军胡晓戚勃岑剑伟马海霞荣辉
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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