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Method for preparing nanofiber scaffold by employing composite cell electrical injection technology

A nanofiber, cell technology, applied in fiber processing, cellulose/protein conjugated man-made filament, conjugated synthetic polymer man-made filament, etc., can solve the problem of inability to form vascularization, affecting cell-cell, cell-scaffold Interaction, low resolution and other problems, to achieve the effect of reducing stent preparation time, facilitating interaction and improving resolution

Inactive Publication Date: 2015-09-02
SHANGHAI UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new technology allows for more uniform distribution of live cells onto a porous structure made from plastic materials called poly(ethylene terephthalate) (PET). By combining two types of liquid droplets into an electric field, these particles move towards each other without being affected or losing their shape during processing. These techniques help improve the accuracy and precision of analyzed biological samples such as blood vessels and tumors.

Problems solved by technology

Technologies described involve creation of nanofibers that serve various applications related to medical science including regenerative therapy (TNT), wound healing, bone ingrowth, cartilage replacement, and dentistry. However, there currently exist challenges associated with these technics because they require expensive equipment like electron microscopic instruments called electronic microscope devices, making them cost-effective only during their use. Additionally, existing techniques often result in nonuniform mixtures leading to reduced viability and functionality of the desired products.

Method used

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  • Method for preparing nanofiber scaffold by employing composite cell electrical injection technology

Examples

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Embodiment 1

[0024] The present invention provides a method for preparing a cell-carrying nanofiber scaffold with composite cell electrospray technology. The nanofiber scaffold is prepared by degradable polymer and cell composite electrospray, including the following steps:

[0025] Step 1: Take 20ml of deionized water and heat it to boiling on a magnetic stirrer, weigh 1.86g of water-soluble and degradable polyvinyl alcohol (PVA) with an electronic balance, and heat the deionized water to boiling under uniform stirring Slowly add to the polymer material to 20g, use water bath heating method to promote the dissolution of the polymer material and stir until fully dissolved. After obtaining a uniform polymer solution, cool for 2 hours and place the solution in a vibrating ball mill for 90 minutes;

[0026] Step 2: after the high-temperature sterilization of the ground polymer solution in a steam pressure cooker at 120° C. for half an hour, the polymer solution was sucked into a 10 ml syringe...

Embodiment 2

[0031] This example is basically the same as Example 1, except that 1.8g of polyethylene oxide (PEO) particles were weighed and slowly added to 8g of deionized water at 70-80°C under stirring, and stirred until fully dissolved to obtain The transparent 18% PEO solution was then ball milled for 90 minutes.

Embodiment 3

[0033] This example is basically the same as Example 1, except that the material is polycaprolactone (PCL) particles, 1g of PCL particles, 3g of dimethylformamide, and 6g of dichloromethane are placed in a 50ml beaker and left to stand. After the PCL is completely dissolved, stir evenly, and finally obtain a PCL solution with a mass volume ratio of 10%.

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Abstract

The invention provides a method for preparing a nanofiber scaffold by employing a composite cell electrical injection technology. The method comprises the following steps: 1, taking a uniform polymer solution, putting the solution into an oscillating ball mill, and grinding for 90 minutes; 2, carrying out high-temperature sterilization on the ground polymer solution in a steam pressure cooker at 120 DEG C for half an hour; 3, digesting 4ml of 25% trypsase for human cells, and then neutralizing with a cell culture fluid with the same volume; and 4 respectively putting a high-molecular polymer solution and a cell suspension liquid on two different electrical injection devices by virtue of the two electrical injection devices contained by electrical injection equipment, and lastingly carrying out electrical injection for 2 hours, so as to obtain the nanofiber scaffold containing cells prepared by a composite electrical injection method. According to the preparation method, the resolution ratio of the cells on the scaffold is significantly improved.

Description

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Claims

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Application Information

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Owner SHANGHAI UNIV
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