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A method for rapidly cultivating early-stage stereotyped tissue culture commercial seedlings of blueberries

A tissue culture and blueberry technology, applied in the directions of horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of uneven quality of seedlings, high production costs of seedlings, long seedling breeding period, etc., and reduce the death rate of axillary buds. , the effect of enhancing tolerance and improving germination rate

Active Publication Date: 2017-03-15
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the low seeding efficiency caused by the pollution of tissue culture materials and the serious death of axillary buds during seeding in the existing tissue culture technology, the long time for inducing rooting in the bottle, the low rooting rate, and the long seedling cultivation period. And after the tissue culture seedlings come out of the nursery, they need to be shaped and shaped, the quality of the seedlings is not uniform, and the production cost of the seedlings is high.

Method used

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  • A method for rapidly cultivating early-stage stereotyped tissue culture commercial seedlings of blueberries
  • A method for rapidly cultivating early-stage stereotyped tissue culture commercial seedlings of blueberries
  • A method for rapidly cultivating early-stage stereotyped tissue culture commercial seedlings of blueberries

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 (the inventive method)

[0035] (1) Field pretreatment of tissue culture materials

[0036] In June, select the blueberry variety 'Brilliant' which is disease-free, grows well, and has a high yield. The individual plant is the mother plant for seed collection. When the length of the new shoot of the mother plant is 10-15 cm, the terminal bud is wiped off, and the terminal bud is 1-2 cm long. When each axillary bud expands to form a spindle-shaped plump axillary bud, the segment with the spindle-shaped plump axillary bud is cut as a tissue culture material.

[0037] (2) Two-step disinfection of isolated axillary buds

[0038] The first step of disinfection: after cleaning the cut tissue culture material in (1), it is sterilized in 0.2% mercury chloride solution for 20 minutes.

[0039] The second step of disinfection: the spindle-shaped plump buds on the tissue culture material sterilized in the first step are cut from the segments as isolated axillary bud...

Embodiment 2

[0052] Embodiment 2 (the inventive method)

[0053] Embodiment 2 is the same as Embodiment 1 except that the following steps are different.

[0054] (1) Field pretreatment of tissue culture materials

[0055] In April, an excellent single plant of the blueberry variety 'Duke' was selected as the mother plant for seed collection.

[0056] (4) Subculture proliferation culture

[0057] Cut the adventitious buds obtained through the induction culture in step (3) into 1.5-2.0 cm segments or small pieces with 1-3 adventitious buds, and inoculate them in the modified WPM+TDZ 1.5mg / L+KT 0.2mg / L +IAA 0.2mg / L+agar 7g / L+sugar 20g / L, pH value of 5.0 subculture medium, subculture culture conditions are: temperature 23 ℃, light intensity 2500LX, light time 8 hours. The subculture cycle is 35 days / time, and the proliferation rate can reach 5.33 times.

[0058] (5) The bottle seedlings are transplanted for the first time

[0059] After 30 days of subculture cultivation in step (4), move ...

Embodiment 3

[0060] Embodiment 3 (the inventive method)

[0061] Embodiment 3 is the same as Embodiment 1 except that the following steps are different.

[0062] (1) Field pretreatment of tissue culture materials

[0063]In October, a blueberry variety 'Legacy' was selected as the mother plant for seed collection, with good growth and high yield.

[0064] (2) Two-step disinfection of isolated axillary buds

[0065] The first step of disinfection: (1) after cleaning the cut tissue culture material, disinfect it in 0.2% mercuric chloride solution for 25 minutes;

[0066] The second step of disinfection: the spindle-shaped plump buds on the tissue culture material sterilized in the first step are cut from the segment as isolated axillary buds, sterilized in 0.1% mercuric chloride solution for 5 minutes, and rinsed with sterile water later used for inoculation;

[0067] (3) Induction culture of isolated axillary buds

[0068] Step (2) Inoculate the sterilized isolated axillary buds into a...

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Abstract

The invention discloses a method for rapidly cultivating early shaping tissue culture commodity seedlings of blueberries, and belongs to the technical field of tissue culture fast propagation and cultivation. The method for rapidly cultivating the early shaping tissue culture commodity seedlings of the blueberries includes: preprocessing tissue culture materials in a field; performing two steps of disinfection on separate auxiliary buds; performing induction cultivating on the separate auxiliary buds; performing subculture multiplication cultivating; performing transplanting twice, wherein transplanting bottle seedlings firstly, and then transplanting rooted seedlings; shaping tissue culture seedlings at an early stage. The method for rapidly cultivating the early shaping tissue culture commodity seedlings of the blueberries solves the technical problems that introduced seedlings are high in pollution rate, explants are serious in death rate, effectively seedling introduction rate is low, production cost is high, the blueberries need to be reshaped after being planted in soil, seedling culture and production cycles are long and the like in common tissue culture of the blueberries, shortens acquisition time of shaping high quality seedlings of a splitting type tree with 3-5 branches by 16.7-31.7 days, improves tissue culture effective seedling introduction rate by 85.39%, reduces production cost, and is high in seedling culture efficiency.

Description

technical field [0001] The invention belongs to the technical field of tissue culture rapid propagation and cultivation, and in particular relates to a method for tissue culture rapid propagation of blueberries and an early shaping method of tissue culture seedlings. Background technique [0002] Blueberry (Vaccinium Spp) is a small berry of a perennial shrub of Ericaceae (Ericaceae) and Vaccinium (Vaccinium). for the 'super fruit'. [0003] my country's research on blueberries began in the 1980s, focusing on the development and utilization of wild blueberry resources in my country, and carried out research on resource investigation, reproduction, processing and efficacy. After the 1990s, Liaoning, Shandong and other places began to introduce foreign commercial varieties for demonstration planting, and to conduct research and application of tissue culture and cutting propagation technology. In recent years, the large-scale planting of blueberries has developed rapidly, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00A01G31/00A01H4/00A01G7/06
CPCA01G2/00A01G7/06A01G24/00A01G31/00A01H4/00A01H4/001
Inventor 吴丽芳杨春梅阮继伟汪国鲜单芹丽曹桦李绅崇屈云慧
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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