Yeast cell short peptide and yeast cell wall polysaccharide synchronous preparation method

A yeast cell wall and yeast technology, which is applied in the field of synchronous preparation of yeast short peptides and yeast cell wall polysaccharides, can solve the problems of increased salt ion content, mannan degradation, and easy hydrolysis, etc., and achieve great economic benefits and environmental protection significance , increase the utilization rate and added value, the effect of mild preparation conditions

Active Publication Date: 2015-09-09
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the acid method will lead to a higher protein content of the obtained product, and the mannan is prone to hydrolysis under acidic conditions; the alkaline method uses a large amount of lye, which will not only cause the degradation of the mannan but also pollute t...

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  • Yeast cell short peptide and yeast cell wall polysaccharide synchronous preparation method

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Embodiment 1

[0034]Example 1: Preparation of zymosan and short peptide

[0035] The simultaneous preparation method of yeast short peptide and yeast cell wall polysaccharide in this embodiment comprises the following steps:

[0036] (1) Saccharomyces cerevisiae was washed with deionized water and then centrifuged to remove impurities, and deionized water was added at a volume ratio of 1:6 to prepare a suspension a, and the neutral protease Neutrase was added to the suspension a at an enzyme amount of 200U / g , under the conditions of pH 7.0 and 55°C, enzymatically hydrolyze and autolyze in a constant temperature deionized water bath shaker for 20 hours, then raise the temperature to 90°C, keep warm for 10 minutes to inactivate the enzyme, and centrifuge to obtain supernatant a and precipitate a respectively;

[0037] (2) using spray drying to dry the supernatant a obtained in step (1) to obtain the yeast short peptide;

[0038] (3) Add deionized water to the precipitate a for centrifugatio...

Embodiment 2

[0043] The simultaneous preparation method of yeast short peptide and yeast cell wall polysaccharide in this embodiment comprises the following steps:

[0044] (1) Saccharomyces cerevisiae was washed with deionized water, centrifuged to remove impurities, and deionized water was added at a volume ratio of 1:4 to prepare a suspension a, and neutral protease Neutrase was added according to the amount of enzyme added at 200U / g, and placed at pH 7.0 and 55 Enzymolysis and autolysis at ℃ for 24 hours, warming up to 90℃, inactivating the enzyme by incubating for 10 minutes, and centrifuging to obtain supernatant a and precipitate a respectively;

[0045] (2) using spray drying to dry the supernatant a obtained in step (1) to obtain the yeast short peptide;

[0046] (3) Add deionized water to the precipitate a for centrifugation to obtain precipitate a1, and then add deionized water to the precipitate a1 at a mass ratio of 1:4 (the mass ratio of precipitate a1 to deionized water is 1...

Embodiment 3

[0051] The only difference between this example and Example 1 is that the volume ratio of Saccharomyces cerevisiae and deionized water in step (1) is 1:5, the dosage of neutral protease Flavorzyme is 300U / g, and when enzymatic hydrolysis is used for autolysis, The temperature is 40°C, the time is 30h, and the pH value is 8.0.

[0052] The yeast short peptide prepared in this example is a milky white powder with a short peptide content of 90.46%; the β-glucan is a milky white powder with a β-glucan content of 94.42%; the yeast mannan is a white powder with a mannan content of was 93.84%.

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Abstract

The invention relates to a preparation method and especially relates to a yeast cell short peptide and yeast cell wall polysaccharide synchronous preparation method. The preparation method comprises carrying out centrifugation cleaning on brewer's yeast as a raw material multiple times, carrying out mixing depression on the cleaned brewer's yeast, carrying out enzymolysis, carrying out centrifugation, collecting a supernatant, carrying out spray drying to obtain the yeast cell short peptide, carrying out precipitation, carrying out high-temperature extraction on the precipitates to obtain mannan and beta-glucan, then carrying out centrifugation to obtain a supernatant and precipitates, carrying out condensation and alcohol precipitation on the supernatant, drying precipitates to obtain mannan, and carrying out high-speed dispersion, enzymatic hydrolysis and high pressure microjet homogeneous treatment on precipitates in the lower layer to obtain beta-glucan. The preparation method has mild conditions, does not adopt strong acid, strong base and organic solvents harmful for environment and realizes large-scale industrial production.

Description

technical field [0001] The invention relates to a preparation method, in particular to a simultaneous preparation method of yeast short peptide and yeast cell wall polysaccharide. Background technique [0002] Saccharomyces cerevisiae (Saccharomyces cerevisiae) is an industrial by-product rich in nutrients. It contains about 50% protein, and the content of eight essential amino acids is very high. The cell wall of Saccharomyces cerevisiae contains 25% to 35% polysaccharides, mainly β-glucan (about 29%) and mannan (about 31%) (Bacon et al, 1969; Fleet et al, 1976; Kath et al , 1999), the main physiological function of β-glucan is to maintain the structure of the cell wall and maintain the normal physiological shape of the cell; while mannan is mainly responsible for cell recognition and interaction with the environment, determining the immune characteristics of yeast ( Ruiz-Herrera, 1992). [0003] The amino acid ratio of yeast protein is coordinated, and short peptide prod...

Claims

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Application Information

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IPC IPC(8): C12P21/06C08B37/02C08B37/00
Inventor 王强刘红芝刘丽石爱民胡晖林伟静刘翠杨颖刘晓永
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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