Three major types of bacteria mixed liquid fermentation, solid yeast feed additive and preparation method thereof

A feed additive, solid fermentation technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of less metabolites, ineffective bacterial agents, etc., to achieve quick effect, fast utilization of drinking water, and easy to attract feeding objects Effect

Inactive Publication Date: 2015-09-16
HARBIN QINGHE TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, most of the products in the market are in the form of dry powder of single bacteria. The advantage is that it is cheap to store and has a large number of bacteria. At the same time, there are few metabolites and a high number of bacteria. Whether it is evenly mixed in the feed and how long it takes to recover in the animal body, Unfavorable factors such as farmers adding drugs, bacterial agents basically do not work

Method used

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  • Three major types of bacteria mixed liquid fermentation, solid yeast feed additive and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Mix liquid fermented feed additives with three types of bacteria, the specific steps are as follows:

[0045] a. Cultivation of mother species: Inoculate 0.5ml of the above-mentioned 5 species of lactic acid bacteria into sterilized test tubes filled with liquid medium, culture anaerobically at 37°C for 48 hours, and prepare the mother species for later use;

[0046] Described liquid culture medium is MRS lactic acid bacteria culture medium and fresh milk culture medium;

[0047] Slope the above-mentioned three kinds of yeast into test tubes filled with sterilized solid medium, culture them at 28°C for 24 hours, and prepare the mother species for later use;

[0048] The slant medium is a PDA medium;

[0049] Put the above-mentioned 6 kinds of spores into the sterilized test tubes filled with solid medium, culture them at 37°C for 12 hours, and keep the mother species for later use;

[0050] The slant medium is LB medium;

[0051]B. Secondary seed cultivation: 5 kinds...

Embodiment 3

[0068] Embodiment 3 (bacillus bacterium liquid fermented product feed additive)

[0069] The purebred culture was the same as in Example 1 with the above-mentioned 6 kinds of bacillus liquid fermented product feed additives.

Embodiment 4

[0071] The bacterial liquid obtained by the method of Example 1 was obtained by the fermentation method of Example 2 to obtain a solid fermented product feed additive.

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Abstract

The invention relates to three major types of bacteria mixed liquid fermentation, solid yeast feed additive and a preparation method thereof and belongs to the field of microbial fermentation. The mixed bacteria yeast is prepared from lactic acid bacteria (five types of strain), saccharomycetes (three types of strain) and bacillus (six types of strain) in a mixing and fermenting mode. The preparation method for the mixed bacteria yeast feed additive includes that firstly, cultivating into mother strain through test tube slant culture medium and test tube fluid medium; secondly, performing shaking table oxygen consumption culture, slant solid culture and anaerobic triangular flask culture, and fermenting into liquid feed additive in a fermentation tank or inoculating to solid fermentation to form the feed additive after fermenting in the fermentation tank. The mixed bacteria yeast feed additive prepared by the method is natural, safe, environmental friendly, rich in metabolite, high in nutritive value and capable of providing the probiotic group needed for the intestines and stomachs of livestock and poultry, preventing diseases, improving the immunity of the livestock and poultry, reducing the harmful gas emission of the livestock and poultry and improving the environment of livestock and poultry houses. The preparation method is simple in culture medium prescription, capable of saving the production cost and lowering the fermentation energy consumption, high in fermentation yield and good in product quality.

Description

technical field [0001] The invention relates to two kinds of feed additives, in particular to liquid fermentation and solid fermentation feed additives of three types of bacteria and a preparation method thereof, belonging to the field of microbial fermentation. Background technique [0002] Mixed-bacteria fermentation refers to a new type of fermentation technology where two or more microorganisms work together to achieve a certain purpose. Mixed bacteria fermentation has a strong uniqueness. It can use the metabolites of one type of bacteria to become nutrients for another type of bacteria, and can obtain some products that cannot be obtained by single fermentation. Fermentation metabolites have an additive effect, and fermentation metabolites Advantages of high utilization rate. [0003] In recent years, the application of microbial preparation products has become more and more extensive. In terms of pharmacy, biopharmaceutical engineering has become the focus of the dev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16
Inventor 程延庆苗晓微梁代华
Owner HARBIN QINGHE TECH
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