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Application of p50 gene in preparation of bladder cancer fighting drug

An anticancer drug and bladder cancer technology, applied in gene therapy, drug combination, antineoplastic drugs, etc., can solve the problem of no p50 tumor inhibitory report

Inactive Publication Date: 2015-09-16
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the tumor suppressor effect of p50 has not been reported

Method used

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  • Application of p50 gene in preparation of bladder cancer fighting drug
  • Application of p50 gene in preparation of bladder cancer fighting drug
  • Application of p50 gene in preparation of bladder cancer fighting drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: p50 gene inhibits bladder cancer T24 cells

[0013] 1. Construction of recombinant expression vector

[0014] 1. Preparation of PCR products

[0015] Using the reverse-transcribed cDNA of T24 cell RNA as a template, the expression primers with restriction sites were designed based on the full-length cDNA sequence of p50 gene (Gene ID: 4971) for PCR amplification. The reaction conditions were: pre-denaturation at 94°C for 3 min, denaturation at 94°C for 30 s, annealing at 60°C for 40 s, extension at 72°C for 3 min, a total of 35 cycles, and incubation at 72°C for 10 min. The primer sequences are listed in Table 1. The PCR product recovery process was carried out strictly according to the instructions of the Gel Extraction Kit of OMEGA Company.

[0016] Table 1 p50 expression primers

[0017]

[0018] 2. Full-length verification of the target gene

[0019] The target gene obtained by PCR was connected to Zero Vector and sent to Beijing Aoke Biological Co...

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Abstract

The invention provides the application of a p50 gene in preparation of cancer fighting drugs, and particularly provides the application of the p50 gene in preparation of a bladder cancer fighting drug. According to the application, transfection of a cancer cell is achieved by establishing an over-expression vector of the p50 gene, and it is indicted by results that, the mRNA of the p50 gene in the cancer cell is greatly increased after transfection; the cancer cell becomes round, the wall attaching performance of the cancer cell becomes poor, the activity of the cancer cell is reduced by 21.8%, and the cancer cell dies finally. According to the application, the gene which can effectively restrain the cancer cell is obtained through screening, and basic data are provided for discovery of novel drug targets and design of tumor drugs.

Description

Technical field: [0001] The invention relates to a new application of the p50 gene, in particular to the application of the p50 gene in the preparation of anticancer drugs. Background technique: [0002] NF-κB is an important transcription factor protein family in mammals, consisting of five members: Rel (cRel), p65 (RelA), RelB, p50 (NF-κB 1), p52 (NF-κB 2). Members of this family combine with subunits to form homologous or heterodimers, bind to specific sequences (-κB sites) on target genes to regulate gene transcription, and fine-tune the expression of different genes. Among them, p50 contains the N-terminal Rel homology domain (Rel homology domain, RHD), does not contain the C-terminal transactivation domain (transactivation domain, TD), and the homodimer formed by it cannot activate gene transcription, often with its The precursor p105 form exists as an inhibitory molecule. [0003] In tumor research, it is generally believed that the NF-κB pathway regulates the trans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P35/00
Inventor 张婷婷张建珍任璐马恩波郑耀武任连生
Owner SHANXI UNIV
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