Chinese magnoliavine fruit lignan single component preparation

A Schisandra lignan, a single technology, used in ether preparation, ether separation/purification, organic chemistry, etc., can solve problems such as resin bed blockage, achieve the effects of lightening load, friendly operating environment, and fewer types of reagents

Inactive Publication Date: 2015-09-16
YANTAI UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The substances adsorbed by the resin are all water-soluble or organic substances with a certain solubility. Schisandra lignans are lipophilic organic substances, which cannot be dissolved and evenly dispersed in the aqueous solution, which will easily lead to blockage of the resin bed, and cannot be effectively processed by resin adsorption. Separation and purification, such

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Processing of raw materials: purchase Schisandra chinensis fruit, dry it in an oven at 60°C to constant weight, then pulverize it into powder with a pulverizer, and collect it for later use.

[0016] (2) Extraction: add 70% ethanol aqueous solution according to the solid-liquid ratio of 1:10 (m / v), extract twice by reflux method, 2 h each time, after the extract is concentrated, add ethyl acetate (1:1, v / v) Extract 3 times.

[0017] (3) Emulsification and dispersion: Concentrate the ethyl acetate extract to extract, and emulsify and disperse with water with the aid of surfactant Tween 40. The ratio is: Schisandra powder: Tween 40: water = 100:3:1000 (m / m / v), the lignan-containing lipophilic extract is uniformly dispersed in the aqueous solution to obtain a uniform and stable solution.

[0018] (4) Preliminary purification by resin adsorption: the extract containing Schisandra lignans after emulsification and dispersion is saturated by LS610B macroporous resin adso...

Embodiment 2

[0025] The same as in Example 1, except that the chromatography medium was changed to ODS-A.

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PUM

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Abstract

The invention discloses a Chinese magnoliavine fruit lignan single component preparation, and belongs to the technical field of medicines, and especially, by addition of a surfactant into a lipophilic Chinese magnoliavine fruit lignan extract for formation of a uniform aqueous solution, macroporous adsorption resin can be effectively used for preliminary purification, subsequent chromatographic purification operation load can be reduced, then ODS-A-HG or ODS-A reversed-phase C18 packing is used for chromatographic purification, and an ethanol aqueous solution is used for gradient elution to obtain schizandrin a, schizandrin b, schisantherin a, schisantherin b, deoxyschizandrin, and schisandrin b single components with the purity of more than 98%. The method has the advantages of being simple in operation, non-pollution, low in cost, less in time consuming, high in yield, easy to enlarge in industry, and the like.

Description

technical field [0001] The invention relates to a preparation method of a single component of schisandra lignan, belonging to the technical field of medicine, in particular adding a surfactant to the lipophilic schisandra lignan extract to form a homogeneous aqueous solution, which can be carried out by macroporous adsorption resin. Preliminary purification, then use ODS-A-HG or ODS-A reversed-phase C18 filler for chromatographic purification, and use ethanol aqueous solution for gradient elution to obtain schisandrin A, schisandrin B, schisandrin methyl with a purity of more than 98% , Schisandra A, Schisandra B single component. Background technique [0002] Schisandra ( Schisandra chinensis (Turcz.) Baill ), named after the combination of sour, sweet, bitter, spicy and salty. The main active ingredient of Schisandra chinensis is lignan, which has the functions of protecting liver, anti-oxidation, anti-inflammatory, anti-cancer and anti-AIDS. Schisandra lignans hav...

Claims

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Application Information

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IPC IPC(8): C07C43/23C07C43/21C07C41/36C07D317/70
CPCC07C41/36C07C2603/36C07D317/70C07C43/23C07C43/21
Inventor 常秀莲徐菡赵镇雷
Owner YANTAI UNIV
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