Chinese magnoliavine fruit lignan single component preparation

Abstract

The invention discloses a Chinese magnoliavine fruit lignan single component preparation, and belongs to the technical field of medicines, and especially, by addition of a surfactant into a lipophilic Chinese magnoliavine fruit lignan extract for formation of a uniform aqueous solution, macroporous adsorption resin can be effectively used for preliminary purification, subsequent chromatographic purification operation load can be reduced, then ODS-A-HG or ODS-A reversed-phase C18 packing is used for chromatographic purification, and an ethanol aqueous solution is used for gradient elution to obtain schizandrin a, schizandrin b, schisantherin a, schisantherin b, deoxyschizandrin, and schisandrin b single components with the purity of more than 98%. The method has the advantages of being simple in operation, non-pollution, low in cost, less in time consuming, high in yield, easy to enlarge in industry, and the like.

Description

technical field [0001] The invention relates to a preparation method of a single component of schisandra lignan, belonging to the technical field of medicine, in particular adding a surfactant to the lipophilic schisandra lignan extract to form a homogeneous aqueous solution, which can be carried out by macroporous adsorption resin. Preliminary purification, then use ODS-A-HG or ODS-A reversed-phase C18 filler for chromatographic purification, and use ethanol aqueous solution for gradient elution to obtain schisandrin A, schisandrin B, schisandrin methyl with a purity of more than 98% , Schisandra A, Schisandra B single component. Background technique [0002] Schisandra ( Schisandra chinensis (Turcz.) Baill ), named after the combination of sour, sweet, bitter, spicy and salty. The main active ingredient of Schisandra chinensis is lignan, which has the functions of protecting liver, anti-oxidation, anti-inflammatory, anti-cancer and anti-AIDS. Schisandra lignans hav...

AI Technical Summary

Problems solved by technology

The substances adsorbed by the resin are all water-soluble or organic substances with a certain solubility. Schisandra lignans are lipophilic organic substances, which cannot be dissolved and evenly dispersed in the aqueous solution, which will easily lead to blockage of the resin bed, and cannot be effectively processed by resin adsorption. Separation and purification, such as application number 200910216886.2 (invention name: a method for enriching and purifying schisandrin A in Schisandra chinensis), and reports by Yin Huang et al. (Yin Tian, ​​Liu Yansheng, Yu Yeling, Tang Xing. Extraction with macroporous resin and purification of total lignans from Schisandra chinensis. Journal of Shenyang Pharmaceutical University, 2007, 24(2): 113-118.)