Chicken FABP1 gene molecular genetic marker related to chicken good production traits and application thereof

A technology of molecular genetic markers and production traits, applied to the application field of broiler chicken breeds

Inactive Publication Date: 2015-09-16
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, there is no report on the application of FABP1 gene SNPs as genetic markers, especially as molecular genetic markers for good slaughtering traits of broiler chickens to screen or predict broiler chicken breeds with good slaughtering traits

Method used

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  • Chicken FABP1 gene molecular genetic marker related to chicken good production traits and application thereof
  • Chicken FABP1 gene molecular genetic marker related to chicken good production traits and application thereof
  • Chicken FABP1 gene molecular genetic marker related to chicken good production traits and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: the cloning of different breed chicken FABP1 gene partial DNA sequence

[0027] 1. Collection and processing of chicken blood: randomly selected disease-free healthy chickens, including 670 Luqin No. 3 chickens and 60 Langya chickens, blood was collected from the underwing vein, and EDTA was used for anticoagulant treatment.

[0028]2. Genomic DNA extraction: DNA was extracted using Tiangen blood DNA extraction kit. Use 200 μL of fresh, frozen or blood with various anticoagulants, add 20 μL proteinase K solution, mix well; then add 200 μL buffer GB, mix well and place at 70°C for 10 minutes; add 200 μL absolute ethanol, shake and mix well At this time, flocculent precipitates will appear; transfer all the obtained solution and flocculent precipitates to the adsorption column CB3 in the collection tube, centrifuge at 12000rpm for 30 seconds and discard the waste liquid; add 500μL buffer GD, centrifuge at 12000rpm for 30 seconds and Discard the waste soluti...

Embodiment 2

[0038] Embodiment 2: Use MboI enzyme to carry out specific enzyme digestion to the PCR product in embodiment 1

[0039] 1. Use MboI enzyme to specifically digest the target gene. The sites of action of these specific enzymes are:

[0040] 5'... ↓ GATC…3’

[0041] 3’…CTAG ↑ …5’

[0042] 2. See Table 3 for the enzyme digestion reaction system.

[0043] Table 3 MboI digestion reaction system

[0044]

[0045] After mixing the solutions of the reaction system according to the above system, place them in a constant temperature water bath at 37°C to react for 3-5 hours, so that the enzyme cleavage reaction can fully proceed, and then carry out subsequent tests.

[0046] 3. The digested products were analyzed by agarose gel electrophoresis. Then classify and summarize all samples according to different enzyme digestion results, and conclude different types.

[0047] There are three kinds of enzyme digestion results, namely complete mutation of SNP site, heterozygous mutati...

Embodiment 3

[0050] Example 3: Diagnostic application of molecular genetic markers prepared by the present invention in polymorphisms in different chicken populations

[0051] 1. Diagnosis in population polymorphism: 670 Luqin No. 3 chickens and 60 Langya chicken FABP1 genes were detected by specific enzyme digestion using the above-mentioned SNP polymorphism detection method.

[0052] 2. Frequency statistical analysis of SNP loci:

[0053] Genotype frequency refers to the ratio of the number of individuals of a certain genotype to the total number of individuals in a population. Paa=Aaa / N, where Paa represents the AA genotype frequency at a certain locus, Aaa represents the number of individuals with the AA genotype in the population, and N is the total number of the detection population.

[0054] Gene frequency refers to the relative ratio of the number of a certain gene to the total number of alleles in a population. The calculation formula can be written as: PA=(2NM+NA al +NA a2 +....

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Abstract

The invention discloses a chicken FABP1 gene molecular genetic marker related to chicken good production traits, which is a polymorphism site on a chicken FABP1 gene mononucleotide polymorphism sequence. The site is a base polymorphism site of which the 83rd position on the FABP1 gene sequence second exon is C or T, and has three genotypes (CC, CT and TT), wherein the TT type is a good slaughter trait molecular genetic marker for meat chickens. The invention also discloses application of the marker in auxiliary screening or prediction of meat chicken species with good slaughter traits. The experiment proves that the polymorphism and productivity of the specific enzyme digestion site are related, and most measurement indexes of the individuals with the genotype of TT are higher than those of the individuals with the genotype of CC or CT, which indicates that the individuals with the genotype of TT can be used as breeding hens for culturing chicken species with good slaughter traits.

Description

technical field [0001] The invention belongs to the field of molecular biology technology, and relates to a molecular genetic marker for excellent slaughtering traits of broiler chickens and its application in assisting screening or prediction of broiler chicken breeds with excellent slaughtering traits. Background technique [0002] The current relatively complete description of molecular genetic markers refers to a certain type of phenotypic characteristics or genetic material that is easy to identify, follows the Mendelian inheritance pattern, has individual specificity or distribution rules, and has germplasm characteristics. Its scope includes: genes or Variation characteristics of genetic material products, morphological variation of chromosomes as carriers of genes or genetic material, variation of genes or genetic material itself, etc. DNA molecular genetic marker technology is a new molecular marker technology, which has been widely used in molecular biology, especi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 林浴霜王丹丹韩海霞李福伟高金波刘玮
Owner SHANDONG UNIV
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