Blood pretreatment liquor

A technology for pretreatment of liquid and blood, applied in the preparation of test samples, etc., can solve problems such as difficult calculation and clear, and achieve the effect of preventing blood coagulation

Active Publication Date: 2015-09-23
NINGBO MEIJING MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above two inventions count white blood cells and red blood cells with a large number in the blood, but count the disease factors with a small number in the blood, such as circulating tumor cells, which is like finding a needle in a haystack. Counting specially designed a blood pretreatment solution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] A blood pretreatment solution, its composition and concentration are as follows: the concentration of sodium chloride is 3g / L, the concentration of potassium dihydrogen phosphate is 0.2g / L, the concentration of dibasic sodium phosphate is 2g / L, and the concentration of calf serum albumin is 100g / L. The concentration is 3%, the ionic detergent high-carbon alcohol concentration is 0.1%, the protein protectant glycerin concentration is 0.5%, the Proclin-300 concentration is 0.01%, and the balance is purified water. When processing, take 7.5ml of fresh blood and add 4.4ml of blood pretreatment solution, fall up and down four or five times, put it in a centrifuge for 10 minutes, discard the supernatant, and keep the cells.

Embodiment 2

[0011] A blood pretreatment solution, its composition and concentration are as follows: the concentration of sodium chloride is 5g / L, the concentration of potassium dihydrogen phosphate is 0.1g / L, the concentration of dibasic sodium phosphate is 1g / L, and the concentration of calf serum albumin is 100g / L. The concentration is 4%, the mass percentage concentration of ion detergent fatty acid or high carbon fatty amine is 0.5%, the mass percentage concentration of protein protection agent galactose (or arabinose or glucose or maltose) is 1.5%, the mass percentage concentration of Proclin-300 The percentage concentration is 0.03%, and the balance is purified water.

Embodiment 3

[0013] A blood pretreatment solution, its composition and concentration are as follows: the concentration of sodium chloride is 8g / L, the concentration of potassium dihydrogen phosphate is 0.15g / L, the concentration of dibasic sodium phosphate is 1.5g / L, and the concentration of calf serum albumin The percentage concentration is 5%, the ionic detergent ethylene oxide or polyethylene glycol mass percentage concentration is 1%, and the protein protection agent is a mixed polysaccharide prepared from galactose and arabinose (or glucose and maltose, galactose and Maltose, glucose and arabinose, etc.), its mass percentage concentration is 3%, Proclin-300 mass percentage concentration is 0.05%, and the balance is purified water. It is better to have a good fusion effect of activity, which can be 1:0.1~10, preferably with a mass ratio of 1:1.

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Abstract

The invention discloses blood pretreatment liquor which includes the following components: sodium chloride, monopotassium phosphate, dibasic sodium phosphate, bovine serium albumin, an ionic detergent, a protein protectant, Proclin-300 and water; the interfacial free energy can be reduced by the ionic detergent, and the interfacial properties of to-be-removed objects and the matrix can be changed, glycerol, active polysaccharides and the biological activity of bovine serium albumin can be fused for function enhancement through like charges repelling, filtering or magnetic adsorption of an adsorption layer. With the utilization of the blood pretreatment liquor provided by the invention, after centrifuging blood, white blood cells, infected factors and target cells and the like are concentrated and purified, and the surface bioactivity of the cells cannot be damaged.

Description

technical field [0001] The invention relates to a treatment liquid for blood treatment, in particular to a blood pretreatment liquid. Background technique [0002] Blood includes serum, white blood cells, red blood cells, and platelets, but many disease factors first affect the changes in blood components, such as circulating tumor cells (circulating tumor cells, CTCs). Tumor cells that enter the peripheral blood of the human body are usually called circulating tumor cells. The analysis of blood components is mainly to analyze and count the white blood cells and red blood cells in the blood. To analyze these components, the blood needs to be pretreated to prevent blood coagulation, material decomposition and cell death. For example, the invention patent application with the patent publication number of CN103743616, Just disclosed the diluent for blood analyzer, its composition comprises sodium chloride, potassium chloride, sodium sulfate, edetate, imidazole, purine, antisep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/34G01N1/40
Inventor 张晓晶沈挺乐飚
Owner NINGBO MEIJING MEDICAL TECH
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