Method for preserving Chinese cabbage root pathogens

Abstract

The invention discloses a method for preserving Chinese cabbage root pathogens, which comprises the steps: 1 fresh Chinese cabbage root pathogenic mycorrhiza is drained off through sterile water after being cleaned, and are sliced into slices to be placed into a self-sealing bag; 2 the slices are pre-frozen for 24 hours in -20 DEG C; 3 the slices are taken out to be dried 48 hours in a vacuum freezer dryer below zero degree in a vacuum state; 4 the slices are rapidly taken out to grind to be mycorrhiza fungus powder, the granularity of mycorrhiza fungus powder is about 50-100 meshes, the mycorrhiza fungus powder is separately placed into small self-sealing bags, and each self-sealing bag is about 10-30g and is closed, and is preserved in room temperature. The method for preserving the Chinese cabbage root pathogens freezes and dries fresh mycorrhiza by utilizing a freezing vacuum drying method, the mycorrhiza which is processed through the method for preserving the Chinese cabbage root pathogens can be preserved for long time in normal temperature, does not need in low temperature preservation condition, and can be made into powder to reduce volume and to conveniently carry, thereby simplifying exchange of pathogenic bacteria between different places. The method for preserving the Chinese cabbage root pathogens is high in pathogenicity of mycorrhiza, and also reduces 90% weight simultaneously.

Description

technical field [0001] The invention relates to the field of strain preservation, in particular to a method for preserving Chinese cabbage clubroot bacteria. Background technique [0002] The preservation of bacterial strains is the basic work of pathogenicity and molecular biology research. Each type of bacterial strain has different characteristics. If it is continuously cultured and passed down, it will cost a lot of manpower and financial resources. Under the influence of biological and biological factors, the biological characteristics of the strains will change, and may even lose their virulence, and may cause the biological characteristics to mutate. Therefore, only the strains can maintain the original Vitality, excellent production performance, and keeping its genetic traits unchanged from the time of isolation or from the beginning of the experiment, can better carry out the pathogenicity and molecular biology experiments of the bacterial source. Since Plasmodium ...

AI Technical Summary

Problems solved by technology

[0004] Since Plasmodium chinensis is a strictly obligate parasite parasitic on the roots of cruciferous vegetables, it cannot be cultured artificially in vitro, which brings great difficulties to study its pathogenic mechanism from the perspective of molecular biology.

The commonly used method for preserving clubroot pathogenic bacteria is: take fresh enlarged root nodules, wash them and store them sealed in a -20°C refrigerator. There are three problems with this preservation method: 1. It requires a low temperature of -20°C, which is no

Images