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In-vitro preservation method of fragaria ananassa germplasm

An in vitro preservation, germplasm technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as adverse effects, and achieve the effect of prolonging the preservation time

Active Publication Date: 2015-10-07
NINGBO ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] According to literature review, the current method of in vitro preservation of strawberry germplasm is generally to select a growth-inhibiting medium, that is, add paclobutrazol, mannitol, abscisic acid and other inhibitors to the medium, and store at room temperature; add these inhibitors to the medium. There may be certain adverse effects after the germplasm resumes growth

Method used

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  • In-vitro preservation method of fragaria ananassa germplasm

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: a kind of method for in vitro preservation of strawberry germplasm, carries out the following steps successively:

[0025] 1) Take healthy "Red Cheek" strawberry stolons without disease spots and galls, put them in a beaker, and rinse with tap water for 1 hour;

[0026] 2), the stolon of step 1) gained is through routine disinfection, promptly 75% (volume %) alcohol disinfection 30 seconds, 0.1% (mass %) mercury liter disinfection 10min, peel off about 0.5mm of strawberry stem tip meristem, and its Inoculated on MS medium for culture; culture conditions: 16 hours of light, light intensity 15-25 μmol m -2 .s -1 , the temperature is 26-28°C; 8 hours of dark cultivation, the temperature is 20-22°C; the above-mentioned light and dark cultivation are carried out alternately;

[0027] The MS medium is: MS basic medium + sugar 30g / l + agar 8g / l, pH is 5.5-6.0.

[0028] 3), when the above-mentioned shoot apex meristem grows to about 0.5cm seedlings, transfer it...

Embodiment 2

[0039] Embodiment 2: " red cheek " strawberry runner in embodiment 1 is renamed " Zhang Ji " strawberry runner; All the other are the same as embodiment 1.

[0040] The results obtained are shown in Table 2 below.

[0041] Table 2

[0042] Seedling growth

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Abstract

The invention discloses an in-vitro preservation method of fragaria ananassa germplasm. The in-vitro preservation method of fragaria ananassa germplasm comprises the following steps in sequence: (1) taking healthy fragaria ananassa creeping stems without disease spot and insect gall, and washing the fragaria ananassa creeping stems with running water; (2) wringing 0.4 to 0.6mm of shoot apical meristems from the fragaria ananassa creeping stems, and inoculating the shoot apical meristems to MS medium for culturing; (3) transferring plantlets to a proliferation medium for culturing after the shoot apical meristems grow to 0.5cm; (4) inoculating adventitious buds into a rooting preservation medium for rooting culturing when the adventitious buds obtained in the step (3) grow to 1.5cm; and (5) putting fragaria ananassa tissue culture plantlets obtained in the step (4) and the rooting preservation medium in a refrigeration box for preserving at 0 to 4 DEG C, culturing under weak light with illumination intensity being 5 to 10 micromoles per square meter in one second. Through the adoption of the method, a series of problems of influence of diseases and insect pests and variety degeneration in the conventional preservation of seed nurseries can be solved.

Description

technical field [0001] The invention relates to a method for in vitro preservation of strawberry germplasm. Background technique [0002] Strawberry (Fragaria ananassa Duch.) belongs to Rosaceae (Rosaceae) Fragaria (Fragaria) perennial evergreen herb, and is an important economic crop widely cultivated in the world. However, the main domestic strawberry varieties are single, and the current species degradation is serious. Therefore, it is necessary to select and breed excellent varieties. The first step in solving the problem of breeding excellent strawberry varieties is to establish a strawberry germplasm resource bank to preserve germplasm economically and effectively. resources to lay the foundation for strawberry breeding. Strawberries generally rely on stolons for vegetative reproduction. Traditional germplasm storage methods require year-round planting and management of strawberry plants. During the storage process, they are not only easily affected by various factors...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张国芳沈岚毛碧增黄坚王芳朱宏芬刘健严成其
Owner NINGBO ACAD OF AGRI SCI
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