Rapid Propagation Method of Uncaria tomentosa

A fast technology of Uncaria chinensis is applied in the field of plant cultivation to achieve the effects of improving survival rate, improving tissue cell division and promoting cell division.

Active Publication Date: 2017-03-15
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the tissue rapid propagation of other species in the genus Uncaria has been reported, but the tissue culture and rapid propagation of Uncaria tomentosa branches as propagation materials have not been reported

Method used

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  • Rapid Propagation Method of Uncaria tomentosa
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  • Rapid Propagation Method of Uncaria tomentosa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A kind of rapid propagation method of uncaria torticola, comprises the following steps:

[0038]Step 1. Make sterile explants of Uncaria toritoides, select the tender stems with joints close to the terminal buds of Uncaria toritoides, remove excess leaves, use a fine brush to gently scrub the surface of the explants under running water, and then cut them into 2cm The long young stem segments with axillary buds were used as sterile explants of Uncaria toriticola. Disinfect the explants of Uncaria torifolia, and the specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the explants with sterile water. Plant three times, then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar with a mass fraction of 1% for 25 minutes, and then rinse the explant continuously with sterilized tea water for 20 seconds .

[0039] Step 2, transfer the sterile explants into th...

Embodiment 2

[0051] A kind of rapid propagation method of uncaria torticola, comprises the following steps:

[0052] Step 1. Make sterile explants of Uncaria toritoides, select the tender stems with joints close to the terminal buds of Uncaria toritoides, remove excess leaves, use a fine brush to gently scrub the surface of the explants under running water, and then cut them into 4cm The long young stem segments with axillary buds were used as sterile explants of Uncaria toriticola. Disinfect the explants of Uncaria torifolia, and the specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the explants with sterile water. Plant three times, then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar with a mass fraction of 1% for 30 minutes, and then rinse the explant continuously with sterilized tea water for 30 seconds .

[0053] Step 2. Transfer the sterile explants into t...

Embodiment 3

[0065] A kind of rapid propagation method of uncaria torticola, comprises the following steps:

[0066] Step 1. Make sterile explants of Uncaria toriticola, select the tender stem section with joints close to the terminal buds of Uncaria toriticola, remove excess leaves, gently scrub the surface of the explant with a fine brush under running water, and then cut it into 3cm The long young stem segments with axillary buds were used as sterile explants of Uncaria toriticola. Disinfect the explants of Uncaria torifolia, and the specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the explants with sterile water. Plant three times, then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar with a mass fraction of 1% for 27 minutes, and then rinse the explant continuously with sterilized tea water for 26 seconds .

[0067] Step 2, transfer the sterile explants into...

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Abstract

The invention discloses a rapid propagation method of Uncaria hirsuta Havil.. The rapid propagation method comprises the following steps: step 1, manufacturing an aseptic explant of Uncaria hirsuta Havil.; step 2, carrying out illumination culturing on the aseptic explant in a first culture medium; step 3, carrying out illumination culturing on the aseptic explant with axillary buds obtained in the step 2 in a second culture medium till roots grow out; and step 4, transplanting the rooted Uncaria hirsuta Havil. seedling into a medium for culturing. According to the rapid propagation method established in the invention, the propagation rate is high, the propagation is rapid, large-area popularization and planting in production are facilitated, and the urgent needs for development of natural resources of Chinese medicinal materials are met.

Description

technical field [0001] The invention relates to the field of plant cultivation, and more specifically, the invention relates to a rapid propagation method of Uncaria tostica. Background technique [0002] Uncaria is Rubiaceae, Chinchonae Uncaria genus, aliased to hang vine and Taiwan vine. It belongs to vines with climbing habit, slender shoots, cylindrical or slightly angular, covered with bristles. Uncaria uncaria is a unique species in my country, mainly distributed in Guangdong, Guangxi, Guizhou, Fujian and Taiwan, China, and grows in thickets by streams and under forests in valleys. The roots and hooked stems of Uncaria plants have high rhynchophylline content, which has the effects of lowering blood pressure, calming, anti-platelet aggregation and anti-thrombosis, and has certain anti-cancer effects. Uncaria uncaria is mostly wild resources, and artificial cultivation mostly is field introduction (including seed sowing, division and cutting), but its germination rate,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 付金娥韦树根马小军潘丽梅白隆华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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