Traditional Chinese medicine bio-preparation of improving immunity of livestock and poultry, preparation method thereof and feed therewith

A technology of biological preparations and immunity, applied in animal feed, animal feed, drug combination, etc., can solve the problems of disproportionate development of animal husbandry, low bioavailability, low technical content, etc., to improve the immunity and production of livestock and poultry Performance, low bioavailability, effect of reduced dosage

Inactive Publication Date: 2015-10-14
BEIJING DABEINONG ANIMAL HEALTH TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional Chinese veterinary drug preparations currently used are generally powders with low technical content, which are directly added to feed and have low bioavailability and poor palatability, which is extremely disproportionate to the development of animal husbandry.

Method used

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  • Traditional Chinese medicine bio-preparation of improving immunity of livestock and poultry, preparation method thereof and feed therewith
  • Traditional Chinese medicine bio-preparation of improving immunity of livestock and poultry, preparation method thereof and feed therewith
  • Traditional Chinese medicine bio-preparation of improving immunity of livestock and poultry, preparation method thereof and feed therewith

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 The role of Enterococcus faecalis (CMGCC No.5092) in the process of fermenting corn flour

[0029] Mix corn flour and water thoroughly at a ratio of 1:0.5, inoculate Enterococcus faecalis CGMCC No.5092 bacterial powder with an inoculation amount of 1%, and add 10 7 / g, the fermentation temperature was 37°C, and the fermentation time was 3 days. The fermentation vessel is a 250mL Erlenmeyer flask, which is then placed in an incubator for fermentation. The blank control was the mixture of corn flour and water (1:0.5) not inoculated with Enterococcus faecalis powder, and the control 2 was the mixture of corn flour and water (1:0.5) inoculated with Bacillus subtilis powder with 1% inoculum.

[0030] Results: Compared with the blank control, the color, consistency and flavor of corn flour were significantly improved after being fermented by Enterococcus faecalis ( figure 1 ), the color, consistency and flavor of the fermented corn flour inoculated with Enterococc...

Embodiment 2

[0031] Embodiment 2 Preparation of oral liquid of the present invention

[0032] Composition of traditional Chinese medicine: 100 parts of Hedyotis diffusa, 100 parts of Scutellaria baicalensis, 50 parts of Astragalus membranaceus, 50 parts of licorice

[0033] Preparation:

[0034] (1) Inoculate Enterococcus faecium (commercially available, ACCC 00661) into MRS medium, and culture it on a shaker at 37°C at 250 rpm to produce a first-grade seed liquid. When the viable bacteria content reaches 1.0×10 8～9 When cfu / ml is transferred to the secondary seed liquid fermenter for expanded cultivation, the inoculum size is 10%, the cultivation temperature is 30°C, the stirring speed is 220rpm, and the live bacteria content of the secondary seed liquid reaches 1.0×10 8～9 When cfu / ml, stop culturing and reserve;

[0035] (2) Take the traditional Chinese medicinal materials in the formula, crush them into 120-mesh medicinal material fine powder, put them into a fermentation tank, add 20...

Embodiment 3

[0039] Embodiment 3 Preparation of powder of the present invention

[0040] Composition of traditional Chinese medicine: 75 parts of Hedyotis diffusa, 50 parts of Scutellaria baicalensis, 100 parts of Astragalus membranaceus, 75 parts of licorice

[0041] Preparation:

[0042] (1) Inoculate Enterococcus faecalis (CGMCC No.5092) into MRS medium, and culture it on a shaker at 36°C at 230rpm to produce first-grade seed liquid. When the viable bacteria content reaches 1.0×10 8～9 When cfu / ml is transferred to the secondary seed liquid fermenter for expanded cultivation, the inoculum size is 8%, the culture temperature is 29°C, the stirring speed is 230rpm, and the viable bacteria content of the secondary seed liquid reaches 1.0×10 8～9 When cfu / ml, stop culturing and reserve;

[0043] (2) Take the traditional Chinese medicinal materials in the formula, crush them into 100-mesh medicinal material fine powder, put them into a fermentation tank, add 15% bran, 10% soybean meal, 0.08% ...

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Abstract

The invention relates to a traditional Chinese medicine bio-preparation of improving the immunity of livestock and poultry, a preparation method thereof and a feed therewith. According to the invention, the traditional Chinese medicine bio-preparation is prepared by combining a traditional Chinese medicine preparation with modern microorganism technologies, wherein the bio-preparation is prepared through the steps of: (1) employing the traditional Chinese medicines comprising oldenlandia diffusa, scutellaria baicalensis, astragalus membranaceus and liquorice as raw materials; (2) crushing the raw materials and adding bran, soybean meal, magnesium sulfate, manganese sulfate, sodium acetate and water to form a fermentation culture medium; (3) adding a bacterial strain culture solution in a certain ratio and performing fermentation; and (4) adding alcohol to a matured solid fermentation product to perform extraction, filtration and concentration to prepare various preparations. The traditional Chinese medicine bio-preparation, prepared through microorganism fermentation and metabolism, can significantly improve the immunity of livestock and poultry, can enhance body immunity, can increase production performance of the livestock and poultry, and can effectively prevent and treat immunosuppressive diseases.

Description

technical field [0001] The invention relates to a traditional Chinese medicine biological preparation for improving the immunity of livestock and poultry, a preparation method thereof and feed, belonging to the field of veterinary medicine and feed. Background technique [0002] The current epidemiological characteristics of animal epidemics are mostly atypical viral diseases, bacterial diseases, mixed infections, and secondary infections. The advantages of traditional Chinese veterinary medicine can be fully utilized in the weak areas of western medicine. Especially with the continuous acceleration of the intensification of my country's aquaculture industry, especially driven by the wave of pursuing green, environmental protection and ecology, traditional Chinese veterinary medicine has gained more and more attention in the aquaculture industry due to its unique advantages in improving immune function. Widespread application. Studying the effect of veterinary drug preparati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/748A23K1/16A61P37/04A61P3/00
Inventor 张静刘澜王安如莫云
Owner BEIJING DABEINONG ANIMAL HEALTH TECH
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