Preparation of fluorescent microsphere immunochromatographic test strip and quantitative detection method

A technology of immunochromatography test paper and fluorescent microspheres, which is applied in the field of biomedical detection, can solve the problems of protein molecules falling off easily again, limited application range, poor ability to resist solution interference, etc., to increase fluorescence stability and fluorescence life, expand Scope and type, the effect of overcoming the easy leakage of dyes
CN104977409AInactive Publication Date: 2015-10-14刘宏飞
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
刘宏飞
Publication Date
2015-10-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method of a fluorescent microsphere immunochromatographic test strip and a quantitative detection method. The fluorescent microsphere immunochromatographic test strip is prepared by taking light emitting nanoparticles prepared by a soap-free emulsion polymerization method as a label and is prepared through an immunochromatographic technology; and then, the fluorescent microsphere immunochromatographic test strip is prepared into a detection card comprising a sample cushion, a glass fibre film, a nitrocellulose film and a piece of absorbent paper, wherein a detection line and a quality control line are fixed on the nitrocellulose film. The quantitative detection method disclosed by the invention comprises the following steps: stimulating by adopting an optimal excitation light source of a fluorescent microsphere, enabling emitted fluorescence to pass through a light filter, collecting, gathering and multiplying an emission spectrum by using a CCD scanning technology or an optical fibre technology, converting into a numerical signal, multiplying the tested fluorescence intensity of a detection line by a correction coefficient, substituting the corrected fluorescence intensity in a pre-set standard curve in a fluoroanalyzer, and then, automatically calculating through the fluoroanalyzer so as to obtain the concentration of an object to be detected in a sample. The detection method disclosed by the invention is high in sensitivity, accurate in quantification and convenient to operate.
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Description

technical field

[0001] The invention relates to the field of biomedical detection, in particular to a preparation method and a quantitative detection method of a fluorescent microsphere immunochromatography test strip. technical background

[0002] In biological and medical testing, immunoassays are often used, including radioimmunoassays, enzyme-linked immunoassays, and immunochromatography. Radioimmunoassay and enzyme-linked immunoassay require expensive equipment, professional operators and complicated operation steps, and it is difficult to obtain test results quickly. Immunochromatography is often used for rapid qualitative or semi-quantitative detection due to its simple operation, rapidity and low cost, such as colloidal gold test strips such as clenbuterol hydrochloride, hepatitis B surface antigen, aflatoxin and luteinizing hormone. At present, the incidence of common infectious diseases such as AIDS, hepatitis B, hepatitis C, and syphilis in my country continues t...

Claims

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