CYP2C9 and VKORC1 gene typing detection reagent kit

Abstract

The invention discloses a CYP2C9 and VKORC1 gene typing detection reagent kit. The CYP2C9 and VKORC1 gene typing detection reagent kit comprises a gene chip and various primers. The gene chip is provided with 4 mutation sites of CYP2C9 and VKORC1 genes and nucleotide sequence probes in normal contrast and complementation with the mutation sites, wherein the probes have the sequences shown in SEQ ID Nos. 1-8 or sequences complementary to the sequences shown in SEQ ID Nos. 1-8; the gene chip is further provided with an DNA sequence marked with biotin points, wherein the DNA sequence is shown in SEQ ID No.9. The primers have the sequences shown in SEQ ID Nos. 10-17. The CYP2C9 and VKORC1 gene typing detection reagent kit provides a CYP2C9 and VKORC1 gene typing joint detection platform, can achieve synchronous joint detection, improve detection specificity, lower the cost and shorten detection time, and has great significance in carrying out CYP2C9 and VKORC1 gene detection and mass screening.

Description

technical field [0001] The invention relates to a kit, in particular to a detection kit for diagnosing CYP2C9 and VKORC1 genes. Background technique [0002] Cytochrome P450 (CYP) is a group of isoenzymes encoded by a group of structurally and functionally related superfamily genes. P450s in mammalian tissues play an important role in the metabolism of drugs and xenobiotics, steroid hormone synthesis, fat-soluble vitamin metabolism, and the conversion of polyunsaturated fatty acids into bioactive molecules. Because it plays a very important role in the biotransformation of exogenous compounds (including drugs and poisons), its activity determines the metabolic rate of drugs, which is directly related to the clearance rate of drugs, and is the first phase enzyme of drug metabolism. , which are also called drug metabolizing enzymes. The composition of P450 enzyme system is complex and controlled by gene diversity, which is called P450 gene superfamily. In the P450 superfami...

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Problems solved by technology

These methods have their own advantages and disadvantages, and need to be improved and standardized

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