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Preparation method and application of a nano-platinum doped/enzyme-modified carbon paste electrode

A technology of carbon paste electrode and nano-platinum, which is applied in the direction of material electrochemical variables, etc., to achieve the effect of simple preparation method, easy surface and small residual current

Inactive Publication Date: 2017-11-07
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The bio-enzyme electrode sensor is currently developed with the characteristics of specificity, stability, fast detection speed, good selectivity, and high sensitivity. It is widely used in the fields of clinical medicine, food, environment, and biological sample detection. The detection of SAM on carbon paste electrode has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Pretreatment of carbon nanotubes and graphite powder: Add 25g of graphite powder, 12g of carbon nanotubes, 25mL of concentrated sulfuric acid, and 13mL of concentrated nitric acid into the reactor, react at a constant temperature of 85°C for 4h, cool to room temperature, filter, and use Wash with deionized water to be neutral, dry in a constant temperature drying oven, and grind to powder to obtain a mixture of pretreated carbon nanotubes and graphite powder;

[0029] (2) Preparation of nano-platinum supported by carbon nanotubes and graphite powder: In the reactor, add 15g of pretreated carbon nanotubes and graphite powder mixture, 41mL of acetone, ultrasonically disperse for 10 min, 0.5g of chloroplatinic acid, stir and dissolve, Then add 2.5g citric acid, stir evenly, adjust pH=8.0, raise the temperature to 120°C for 22 hours, cool to room temperature, filter, wash with deionized water until neutral, dry in a constant temperature drying oven, and grind to powder ...

Embodiment 2

[0033] (1) Pretreatment of carbon nanotubes and graphite powder: In the reactor, add 15g graphite powder, 4g carbon nanotubes, 14mL concentrated sulfuric acid, 5mL concentrated nitric acid, react at a constant temperature of 90°C for 3h, cool to room temperature, filter, and use Wash with deionized water to be neutral, dry in a constant temperature drying oven, and grind to powder to obtain a mixture of pretreated carbon nanotubes and graphite powder;

[0034] (2) Preparation of nano-platinum supported by carbon nanotubes and graphite powder: In the reactor, add 13g of pretreated carbon nanotubes and graphite powder mixture, 45mL of acetone, ultrasonically disperse for 10 min, 1.0g of chloroplatinic acid, stir and dissolve, Then add 3.0g citric acid, stir evenly, adjust pH=7.5, raise the temperature to 120°C for 20 hours, cool to room temperature, filter, wash with deionized water until neutral, dry in a constant temperature drying oven, and grind to powder , that is, a mixtur...

Embodiment 3

[0038] (1) Pretreatment of carbon nanotubes and graphite powder: In the reactor, add 10g graphite powder, 6g carbon nanotubes, 13mL concentrated sulfuric acid, 7mL concentrated nitric acid, react at a constant temperature of 80°C for 5h, cool to room temperature, filter, and use Wash with deionized water to be neutral, dry in a constant temperature drying oven, and grind to powder to obtain a mixture of pretreated carbon nanotubes and graphite powder;

[0039] (2) Preparation of nano-platinum supported by carbon nanotubes and graphite powder: In the reactor, add 7g of pretreated carbon nanotubes and graphite powder mixture, 15mL of acetone, ultrasonically disperse for 10 min, 0.6g of chloroplatinic acid, stir and dissolve, Then add 1.0g citric acid, stir evenly, adjust pH=8.5, raise the temperature to 120°C for 24 hours, cool to room temperature, filter, wash with deionized water until neutral, dry in a constant temperature drying oven, and grind to powder , that is, a mixture...

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Abstract

The invention discloses / a preparation method and application of a nano-platinum-doped / enzyme-modified carbon paste electrode, which is characterized in that: the carbon paste electrode is doped with nano-platinum ions, and 1-ethyl-3-methylimidazole tetra Fluoroborate is used as an adhesive to prepare a carbon paste electrode by mixing carbon nanotubes and graphite powder, and then modify the carbon paste electrode with methyltransferase to obtain a nano-platinum-doped / enzyme-modified carbon paste electrode. Compared with ordinary carbon paste electrodes, the conductivity is improved by 1~2 times, and the electrochemical window is wide, the preparation method is simple, the cost is low, the surface is easy to renew, and the residual current is small; the electrode is used to quickly detect SAM in the sample, and the method has high sensitivity. Good selectivity, short response time, less interference, superior to other detection methods, is a simple, fast, convenient and easy SAM determination method, the fixed methyltransferase electrode sensor prepared by the application has low cost, simple preparation process, specific It has good performance and has the potential to realize automatic on-site determination.

Description

technical field [0001] The invention relates to a preparation method of an electrochemical sensor, in particular to a preparation method and application of an electrochemical sensor for modifying a carbon paste electrode with methyltransferase for detecting S-adenosylmethionine (SAM). Background technique [0002] Carbon paste electrode is a type of electrode made by mixing conductive carbon materials, such as graphite powder, with a hydrophobic binder to form a paste, which is then coated on the surface of the electrode rod or filled into the electrode tube. . Due to the advantages of non-toxicity, wide electrochemical window, simple preparation method, low cost, easy surface renewal, and low residual current, carbon paste electrodes have been widely used in electrochemical analysis, biosensor preparation, environmental detection, and food and drug analysis. However, carbon paste electrodes also have some disadvantages, such as poor conductivity, low sensitivity, and poor ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30
Inventor 李慧芝许崇娟杨春霞
Owner UNIV OF JINAN
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