Device and method for measuring column capacity of chromatographic column

Abstract

The invention relates to a device and method for quickly measuring column capacity of a chromatographic column, wherein a special flow slowing device and matched components are used to achieve the purpose of quick and convenient measurement; the device is sequentially connected with a buffer solution storage tank, a pump, the flow slowing device, a sample introduction injector, a guard column, a to-be-measured chromatographic column, a detector and a liquid waste gathering tank through a liquid flow-through pipeline; the pump, the sample introduction injector and the detector are connected with a terminal through data lines, the sample introduction injector enters a mobile phase, the mobile phase enters the flow slowing device through the thrust of the pump to remove the pulse brought by the pump, enters the chromatographic column and is detected by the detector after coming out, and then a chromatogram map is drawn through calculation; if sample amount is not excessive during entering, the peak shape should be relatively symmetric, and sampling concentration can be increased at this time; if the peak shape is obviously tailed, the column capacity is achieved or exceeded, the chromatographic column can be washed at this time, and the sampling concentration can be gradually decreased to be closed to the column capacity.

Description

technical field [0001] The invention belongs to the field of ion chromatography, especially the technical field that needs to determine the highest sample amount that a specific chromatographic column can bear. Background technique [0002] Column capacity, also called column load, refers to the maximum sample volume allowed without affecting the separation efficiency of the column. The amount of sample that any chromatographic column can bear is limited. When the amount of sample exceeds a certain limit, the chromatographic column is overloaded, the chromatographic peak becomes wider, or even seriously deformed. The column efficiency of the chromatographic column increases rapidly with the increase of sample amount. decline. Therefore, the column capacity is an important parameter to express the characteristics of the column. At present, there are two methods for testing the column capacity of ion chromatography columns. One is to refer to the test method for the exchange...

AI Technical Summary

Problems solved by technology

At present, there are two methods for testing the column capacity of ion chromatography columns. One is to refer to the test method for the exchange capacity of ion exchange resins. This method ignores the ions remaining in the resin voids, and is quantified by titration, and the operation process is relatively complicated.

Another method to determine the column capacity through the change of peak shape is to gradually increase the injection volume and determine the column capacity through the change of the spectrum. This method is greatly affected by the environmental conditions and instrument status, and due to the pressure It is difficult to accurately judge the tolerance prediction, and the pump that provides the flow rate is pulsating, and the sudden change of the pulsating pressure is likely to cause the problem of excessive pressurization and pressure fluctuations of the stationary phase, and even cause the stationary phase to damage to

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