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For the determination of analyte homologues

A complex, hydroxyvitamin technology, used in biological testing, analytical materials, measuring devices, etc., can solve problems such as difficulty in obtaining antibodies

Active Publication Date: 2019-04-09
SIEMENS HEALTHCARE DIAGNOSTICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some of the issues surrounding the measurement of substantially equimolar amounts of analyte congeners include, for example, difficulty in obtaining antibodies that bind analyte congeners equally, and unequal release of analyte congeners bound by endogenous binding substances

Method used

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  • For the determination of analyte homologues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0107] Materials in the following experiments were purchased from Sigma-Aldrich Chemical Corporation (St. Louis MO) or Fluka Chemical Corporation (Milwaukee WI) unless otherwise noted. Parts and percentages disclosed herein are on a weight / volume basis unless otherwise indicated.

[0108] definition :

[0109] mg = milligram

[0110] g = gram

[0111] ng = nanogram

[0112] mL = milliliter

[0113] µL = microliter

[0114] µmol = micromole

[0115] ℃= degrees Celsius

[0116] min = minute

[0117] sec = seconds

[0118] hr = hour

[0119] w / v = weight / volume

[0120] v / v = volume / volume

[0121] EDA = ethylenediamine

[0122] EDAC = N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride

[0123] sulfoNHS or SNHS = sulfo N-hydroxysuccinimide

[0124] TLC = thin layer chromatography

[0125] HPLC = high performance liquid chromatography

[0126] EDTA = ethylenediaminetetraacetic acid salt

[0127] PEG = polyethylene glycol

[0128] EtOAc = ethyl acetate...

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Abstract

The method includes adjusting the contribution of one of the two analyte congeners to the amount of a signal obtained in an assay for determining the total amount of the two analyte congeners in the sample. Using a non-assay receptor that has greater binding affinity for either of the two analyte homologues, the contribution of either to the magnitude of the signal is modulated. The amount of non-assay receptors is sufficient to effect modulation of the contribution of the analyte homologue to the signal. An assay for determining the total amount of two analyte homologues is performed, wherein the assay utilizes at least one assay antibody.

Description

[0001] This application claims the benefit of non-provisional application US Serial No. 13 / 861,422 filed April 12, 2013. The entire contents of the aforementioned applications are expressly incorporated herein by reference. Background technique [0002] The present invention relates to compositions, methods and kits for accurately determining the total amount of an analyte congener in a sample suspected of containing the analyte congener. [0003] Many small molecule compounds or haptens such as, for example, drugs and vitamins, exist as homologues, each of which contributes directly or through the intermediary of one or more of their metabolites to the total biological Contribute to the learning function. In order to obtain an accurate measurement of the total amount of active analyte, it is important to measure substantially equimolar amounts of the homologous forms, because, in the case of unequal amounts of the homologous forms, the signal obtained in the assay is distort...

Claims

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Application Information

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IPC IPC(8): G01N33/82
CPCG01N33/54313G01N33/82
Inventor 魏铁全W.贝兹克E.加西亚
Owner SIEMENS HEALTHCARE DIAGNOSTICS INC