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Application of sepulturin A in preparing myocardial preservation medicine

A kind of sepulturina, 1. Sepulturina technology, applied in the application field in the preparation of cardioprotective drugs

Inactive Publication Date: 2015-11-25
潘光贤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is no report about the cardioprotective activity of this compound

Method used

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  • Application of sepulturin A in preparing myocardial preservation medicine
  • Application of sepulturin A in preparing myocardial preservation medicine
  • Application of sepulturin A in preparing myocardial preservation medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: Separation, preparation and structure confirmation of SepulturinA

[0010] The preparation method of Sepulturin A is the same as that reported in the literature (Hydroxyclerodanes from Salviashannoni, J. Nat. Prod., 2013, 76, 1970-1975).

[0011] Confirmation of structure: colorless crystal, melting point 252-254°C, molecular formula C 20 h 22 o 7 , with an unsaturation of 10. H NMR spectrum data δ H (ppm, DMSO-d 6 , 500MHz): H-1(3.27, d, J=3.5), H-2(3.51, m), H-3(2.33, ddd, J=15.0, 5.0, 1.5), H-3(1.81, ddd , J=15.5, 12.5, 2.5), H-4 (2.59, dd, J=12.5, 5.0), H-6 (1.27, ddd, J=11.0, 3.0, 1.0), H-6 (1.59, m) , H-7 (2.06, m), H-7 (1.60, m), H-8 (2.57, m), H-11 (2.88, dd, J=16.0, 7.5), H-11 (2.04, m ), H-12 (5.61, dd, J=8.5, 1.5), H-14 (6.30, m), H-15 (7.37, t, J=1.5), H-16 (7.29, m), H- 19(4.10, d, J=8.5), H-19(4.16, dd, J=8.5, 2.0), H-20(1.19, s); C NMR data δ C (ppm, DMSO-d 6 , 150Hz): 56.0 (CH, 1-C), 58.7 (CH, 2-C), 19.6 (CH 2 , 3-C), 35.8(CH, 4-C),...

Embodiment 2

[0012] Embodiment 2: The pharmacological action test of SepulturinA

[0013] 1. Materials and Instruments

[0014] Neonatal 1-3 day old SD rat suckling mice (SPF grade), male or female, were provided by the Experimental Animal Center of Guangxi Medical University (production license for experimental animals: SCXK Gui 2009-0002). SepulturinA (purity ≥ 98%) is self-made. Fetal bovine serum was purchased from HyClone Company, DMEM medium was purchased from GIBCO Company, dimethyl sulfoxide (DMSO) and 0.25% trypsin (containing EDTA) were purchased from Solarbio Company, 5-bromodeoxyuridine (5-Brdu), Tetramethylazolium salt (MTT) was purchased from Sigma Company in the United States, the LDH test box was purchased from Nanjing Jiancheng Bioengineering Research Institute, the NOS kit (type) was purchased from Nanjing Jiancheng Bioengineering Research Institute, and the ATPase kit (Nanjing Jiancheng Bioengineering Research Institute) was purchased. Engineering Research Institute), ...

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Abstract

The invention discloses an application of sepulturin A in preparing a myocardial preservation medicine, belonging to the field of medicines. The research shows that sepulturin A can enable the viability of myocardial cells damaged by H2O2 stress to be obviously increased, and therefore, the sepulturin A can be further researched and developed to be prepared into the myocardial preservation medicine.

Description

technical field [0001] The present invention relates to a new application of compound Sepulturin A, in particular to the application of Sepulturin A in the preparation of cardioprotective drugs. Background technique [0002] ElihúBautista et al. isolated and purified the compound SepulturinA for the first time, and published the results in the famous Journal of Natural Products (Hydroxyclerodanes from Salviashannoni, J.Nat.Prod., 2013, 76, 1970-1975). [0003] At present, there is no report about the cardioprotective activity of this compound. Contents of the invention [0004] The object of the present invention is to provide a medical application of SepulturinA. [0005] The above-mentioned purpose is achieved through the following technical solutions: [0006] The application of SepulturinA in the preparation of cardioprotective medicine, the chemical structural formula of SepulturinA is as follows, [0007] detailed description [0008] Below in conjunction wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P9/00
Inventor 潘光贤
Owner 潘光贤