A Method for Extracting Fluorescence Spectrum Information

Abstract

The invention discloses a method for extracting fluorescence spectrum information. The traditional two-dimensional correlation analysis relies on the experience of the analyst to identify different substances. The main problem solved by the method of the present invention is how to raise the traditional perceptual knowledge to a rational and accurate quantitative expression. The invention uses the area enclosed by the specific contour lines on the correlation diagram to discuss the scientific expression method of the best characterization parameter for evaluating the material information, and includes the following steps: step 1, acquisition of dynamic spectrum; step 2, preprocessing of spectrum; The third step is to synchronize the drawing of the contour map; the fourth step is to calculate the area of ​​the contour map, so as to quantify the map. The method of the invention can widely apply the two-dimensional correlation fluorescence spectrum analysis technology to the field of rapid quality analysis of food and agricultural products containing complex systems, with low detection cost, short analysis time and non-destructive detection.

Description

Technical field [0001] The invention relates to a method for extracting fluorescence spectrum information, in particular to a method for extracting two-dimensional correlation fluorescence spectrum information based on contour area. Background technique [0002] Two-dimensional correlation analysis technology expands one-dimensional spectral signals into two-dimensional space for analysis, improves the resolution of the spectrum, simplifies the identification method of complex spectra with multiple overlapping peaks, and provides the correlation between the changes of the signal peaks , To identify and study intra- and intermolecular interactions. There are many ways to display two-dimensional correlation maps. Among them, the most comprehensive and classic display method is the three-dimensional map form, which can load all the information of the sample, but it is not intuitive. The form of the contour map solves the problem of the intuitiveness of the three-dimensional map. It...

AI Technical Summary

Problems solved by technology

There are many ways to display the two-dimensional correlation map, among which the largest, most complete and most classic display method is the three-dimensional map form, which can load all the information of the sample, but lacks intuition

[0004] Most of the methods for analyzing spectra in the prior art rely on the experience of analysts to identify different substances, rather than the results of obtaining spectral information based on specific values, and do not realize the expression of spectra from non-quantitative to quantitative, which reduces the Accuracy and correctness of subsequent model building

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