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Chitosan microparticles with shapes and sizes identical to lurid cells and preparation method of chitosan microparticles

A technology of red blood cells and microparticles, applied in the field of chitosan microparticles with the shape and size of red blood cells and its preparation, to achieve the effects of avoiding instrument calibration errors, high proximity, and high consistency

Active Publication Date: 2015-12-02
四川迈可隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because chitosan has the characteristics of unique biological activity, good biocompatibility and easy degradation, it has good application prospects in the fields of biomedicine and biochemistry. At present, there are spherical chitosan particles with a size equivalent to red blood cells. report (CN104792688A), but there is no report of chitosan microparticles that have the same shape and size as red blood cells. If chitosan microparticles with high consistency of depression and good size uniformity can , it is of great significance to improve the use value of chitosan microparticles and expand its application in the fields of biomedicine and biochemistry

Method used

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  • Chitosan microparticles with shapes and sizes identical to lurid cells and preparation method of chitosan microparticles
  • Chitosan microparticles with shapes and sizes identical to lurid cells and preparation method of chitosan microparticles
  • Chitosan microparticles with shapes and sizes identical to lurid cells and preparation method of chitosan microparticles

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Embodiment 1

[0046] In the present embodiment, the preparation method of chitosan microparticles with red blood cell shape and size is as follows:

[0047] (1) Preparation of spray liquid and receiver liquid

[0048] Preparation of spray liquid: add water-soluble chitosan and water-soluble surfactant PluronicF127 into deionized water and stir until the two are completely dissolved to form a solution, then add diffusion non-solvent DMSO and volatile non-solvent ethanol to the solution and Stir and mix to form a mixed solution, adjust the pH value of the mixed solution to 6.3 with 1mol / L sodium hydroxide aqueous solution, centrifuge the mixed solution after adjusting the pH value at a speed of 8000r / min for 10min, take the supernatant and filter Obtain spray liquid; In the mixed liquid, the massfraction of water-soluble chitosan is 2%, the massfraction of PluronicF127 is 1%, the volumefraction of DMSO is 30%, and the volumefraction of ethanol is 20%;

[0049] Preparation of receiving soluti...

Embodiment 2

[0056] In the present embodiment, the preparation method of chitosan microparticles with red blood cell shape and size is as follows:

[0057] (1) Preparation of spray liquid and receiver liquid

[0058] Preparation of spray liquid: add water-soluble chitosan and water-soluble surfactant PluronicF127 into deionized water and stir until the two are completely dissolved to form a solution, and then add diffusion non-solvent DMSO and volatile non-solvent ethanol to the solution And stir and mix to form a mixed solution, adjust the pH value of the mixed solution to 6.3 with 1mol / L aqueous sodium hydroxide solution, centrifuge the mixed solution after adjusting the pH value at a speed of 8000r / min for 10min, and take the supernatant Filter to obtain spray liquid; in the mixed liquid, the mass fraction of water-soluble chitosan is 2%, the mass fraction of PluronicF127 is 1%, the volume fraction of DMSO is 30%, and the volume fraction of ethanol is 20%;

[0059] Preparation of recei...

Embodiment 3

[0066]In the present embodiment, the preparation method of chitosan microparticles with red blood cell shape and size is as follows:

[0067] (1) Preparation of spray liquid and receiver liquid

[0068] Preparation of spray liquid: add water-soluble chitosan and water-soluble surfactant PluronicF127 into deionized water and stir until the two are completely dissolved to form a solution, then add diffusion non-solvent DMSO and volatile non-solvent ethanol to the solution and Stir and mix to form a mixed solution, adjust the pH value of the mixed solution to 6.3 with 1mol / L sodium hydroxide aqueous solution, centrifuge the mixed solution after adjusting the pH value at a speed of 8000r / min for 10min, take the supernatant and filter Obtain spray liquid; In the mixed liquid, the massfraction of water-soluble chitosan is 2%, the massfraction of PluronicF127 is 1%, the volumefraction of DMSO is 30%, and the volumefraction of ethanol is 20%;

[0069] Preparation of receiving solutio...

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Abstract

The invention provides chitosan microparticles with shapes and sizes identical to lurid cells. The microparticles consist of chitosan and terephthalaldehyde which are subjected to crosslinking reaction; the microparticles are in the shape of a flat disc with a recessed surface; diameters of the discs are 8-10 micrometers; and a variable coefficient value of the microparticles is 5-7%. The preparation method comprises the following steps of (1) preparing jetting fluid and receiving fluid; (2) preparing the chitosan microparticles with the shapes and the sizes identical to the lurid cells by using solvent in a process of diffusing the solvent by evaporation and induction; and (3) washing. The shapes and the sizes of the chitosan microparticles are identical to those of the lurid cells, dimensional homogeneity is good, consistency degree of recessing is high, and special requirements in the field of biomedical laboratory science and biochemistry are met. The chitosan microparticles are particularly suitable for the fields of medicine coating or photographic developers used for medicine conveying or medical imaging, and are also suitable for replacing real lurid cells and serving as standard substances for calibrating hematology analyzers.

Description

technical field [0001] The invention belongs to the field of chitosan microparticles, in particular to a chitosan microparticle with the shape and size of red blood cells and a preparation method thereof. Background technique [0002] Traditional polymer microparticles are mainly spherical, but non-spherical polymer microparticles, especially red blood cell-shaped polymer microparticles, have more advantages in biomedicine and biochemical applications. Compared with spherical polymer microparticles, non-spherical polymer microparticles are less likely to be phagocytized and internalized by macrophages (PharmaceuticalResearch.2009, 26(1), 244), and are better than microspheres or even nanospheres. The targeting effect (Journal of Controlled Release.2007, 121 (1), 3), can avoid being filtered by the spleen, thereby prolonging its half-life in the body (Nature Materials. 2009, 8 (1), 15). Microparticles in the shape of red blood cells can replace red blood cells for the calibr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K49/00G01N33/48G01N1/28C08J3/24C08L5/08
Inventor 巨晓洁王小雪褚良银谢锐汪伟刘壮
Owner 四川迈可隆生物科技有限公司
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