Chitosan microparticles with shape and size of red blood cells and preparation method thereof

A technology of red blood cells and chitosan, which is applied in the preparation of test samples, medical preparations of inactive ingredients, preparations for in vivo experiments, etc., to achieve good monodispersity, avoid instrument calibration errors, and long half-life. Effect

Active Publication Date: 2017-12-15
四川迈可隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because chitosan has the characteristics of unique biological activity, good biocompatibility and easy degradation, it has good application prospects in the fields of biomedicine and biochemistry. At present, there are spherical chitosan particles with a size equivalent to red blood cells. report (CN104792688A), but there is no report of chitosan microparticles that have the same shape and size as red blood cells. If chitosan microparticles with high consistency of depression and good size uniformity can , it is of great significance to improve the use value of chitosan microparticles and expand its application in the fields of biomedicine and biochemistry

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  • Chitosan microparticles with shape and size of red blood cells and preparation method thereof
  • Chitosan microparticles with shape and size of red blood cells and preparation method thereof
  • Chitosan microparticles with shape and size of red blood cells and preparation method thereof

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Embodiment 1

[0046] In the present embodiment, the preparation method of chitosan microparticles with red blood cell shape and size is as follows:

[0047] (1) Preparation of spray liquid and receiver liquid

[0048] Preparation of spray liquid: Add water-soluble chitosan and water-soluble surfactant Pluronic F127 into deionized water and stir until the two are completely dissolved to form a solution, and then add diffusion non-solvent DMSO and volatile non-solvent ethanol to the solution And stir and mix to form a mixed solution, adjust the pH value of the mixed solution to 6.3 with 1mol / L aqueous sodium hydroxide solution, centrifuge the mixed solution after adjusting the pH value at a speed of 8000r / min for 10min, and take the supernatant Filter to obtain spray liquid; in the mixed liquid, the mass fraction of water-soluble chitosan is 2%, the mass fraction of Pluronic F127 is 1%, the volume fraction of DMSO is 30%, and the volume fraction of ethanol is 20%;

[0049] Preparation of rec...

Embodiment 2

[0056] In the present embodiment, the preparation method of chitosan microparticles with red blood cell shape and size is as follows:

[0057] (1) Preparation of spray liquid and receiver liquid

[0058] Preparation of spray liquid: Add water-soluble chitosan and water-soluble surfactant Pluronic F127 into deionized water and stir until the two are completely dissolved to form a solution, and then add diffusion non-solvent DMSO and volatile non-solvent to the solution Ethanol and stirring and mixing to form a mixed solution, adjust the pH value of the mixed solution to 6.3 with 1mol / L aqueous sodium hydroxide solution, centrifuge the mixed solution after adjusting the pH value at a speed of 8000r / min for 10min, and take the supernatant Liquid filtration obtains spray liquid; In described mixed solution, the massfraction of water-soluble chitosan is 2%, the massfraction of Pluronic F127 is 1%, the volumefraction of DMSO is 30%, and the volumefraction of ethanol is 20%;

[0059...

Embodiment 3

[0066]In the present embodiment, the preparation method of chitosan microparticles with red blood cell shape and size is as follows:

[0067] (1) Preparation of spray liquid and receiver liquid

[0068] Preparation of spray liquid: Add water-soluble chitosan and water-soluble surfactant Pluronic F127 into deionized water and stir until the two are completely dissolved to form a solution, and then add diffusion non-solvent DMSO and volatile non-solvent ethanol to the solution And stir and mix to form a mixed solution, adjust the pH value of the mixed solution to 6.3 with 1mol / L aqueous sodium hydroxide solution, centrifuge the mixed solution after adjusting the pH value at a speed of 8000r / min for 10min, and take the supernatant Filter to obtain spray liquid; in the mixed liquid, the mass fraction of water-soluble chitosan is 2%, the mass fraction of Pluronic F127 is 1%, the volume fraction of DMSO is 30%, and the volume fraction of ethanol is 20%;

[0069] Preparation of rece...

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Abstract

The invention provides a chitosan microparticle with the shape and size of red blood cells, the microparticle is formed by cross-linking reaction of chitosan and terephthalaldehyde, the microparticle is in the shape of a flat disc with one side depressed, and the disc The diameter of the microparticles is 8-10 μm, and the coefficient of variation of the micro-particles is 5-7%. The preparation method is as follows: (1) preparing spray liquid and receiving liquid; (2) adopting solvent volatilization to induce solvent diffusion process to prepare chitosan microparticles with the shape and size of red blood cells; (3) washing. The shape and size of chitosan microparticles in the present invention are equivalent to those of red blood cells, and have good size uniformity and high consistency of depression, which meet the special needs of biomedicine, biochemistry and other fields, and are especially suitable for encapsulating drugs Or the imaging agent is used in the field of drug delivery or medical imaging, and is also suitable for replacing real red blood cells as a standard for calibrating blood cell analyzers.

Description

technical field [0001] The invention belongs to the field of chitosan microparticles, in particular to a chitosan microparticle with the shape and size of red blood cells and a preparation method thereof. Background technique [0002] Traditional polymer microparticles are mainly spherical, but non-spherical polymer microparticles, especially red blood cell-shaped polymer microparticles, have more advantages in biomedicine and biochemical applications. Compared with spherical polymer microparticles, non-spherical polymer microparticles are less likely to be phagocytized and internalized by macrophages (PharmaceuticalResearch.2009, 26(1), 244), and are better than microspheres or even nanospheres. The targeting effect (Journal of Controlled Release.2007, 121(1), 3), can avoid being filtered by the spleen, thereby prolonging its half-life in vivo (Nature Materials.2009, 8(1), 15). Microparticles in the shape of red blood cells can replace red blood cells for the calibration o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/36A61K49/00G01N33/48G01N1/28C08J3/24C08L5/08
Inventor 巨晓洁王小雪褚良银谢锐汪伟刘壮
Owner 四川迈可隆生物科技有限公司
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