Chitosan microparticles with shape and size of red blood cells and preparation method thereof
A technology of red blood cells and chitosan, which is applied in the preparation of test samples, medical preparations of inactive ingredients, preparations for in vivo experiments, etc., to achieve good monodispersity, avoid instrument calibration errors, and long half-life. Effect
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Embodiment 1
[0046] In the present embodiment, the preparation method of chitosan microparticles with red blood cell shape and size is as follows:
[0047] (1) Preparation of spray liquid and receiver liquid
[0048] Preparation of spray liquid: Add water-soluble chitosan and water-soluble surfactant Pluronic F127 into deionized water and stir until the two are completely dissolved to form a solution, and then add diffusion non-solvent DMSO and volatile non-solvent ethanol to the solution And stir and mix to form a mixed solution, adjust the pH value of the mixed solution to 6.3 with 1mol / L aqueous sodium hydroxide solution, centrifuge the mixed solution after adjusting the pH value at a speed of 8000r / min for 10min, and take the supernatant Filter to obtain spray liquid; in the mixed liquid, the mass fraction of water-soluble chitosan is 2%, the mass fraction of Pluronic F127 is 1%, the volume fraction of DMSO is 30%, and the volume fraction of ethanol is 20%;
[0049] Preparation of rec...
Embodiment 2
[0056] In the present embodiment, the preparation method of chitosan microparticles with red blood cell shape and size is as follows:
[0057] (1) Preparation of spray liquid and receiver liquid
[0058] Preparation of spray liquid: Add water-soluble chitosan and water-soluble surfactant Pluronic F127 into deionized water and stir until the two are completely dissolved to form a solution, and then add diffusion non-solvent DMSO and volatile non-solvent to the solution Ethanol and stirring and mixing to form a mixed solution, adjust the pH value of the mixed solution to 6.3 with 1mol / L aqueous sodium hydroxide solution, centrifuge the mixed solution after adjusting the pH value at a speed of 8000r / min for 10min, and take the supernatant Liquid filtration obtains spray liquid; In described mixed solution, the massfraction of water-soluble chitosan is 2%, the massfraction of Pluronic F127 is 1%, the volumefraction of DMSO is 30%, and the volumefraction of ethanol is 20%;
[0059...
Embodiment 3
[0066]In the present embodiment, the preparation method of chitosan microparticles with red blood cell shape and size is as follows:
[0067] (1) Preparation of spray liquid and receiver liquid
[0068] Preparation of spray liquid: Add water-soluble chitosan and water-soluble surfactant Pluronic F127 into deionized water and stir until the two are completely dissolved to form a solution, and then add diffusion non-solvent DMSO and volatile non-solvent ethanol to the solution And stir and mix to form a mixed solution, adjust the pH value of the mixed solution to 6.3 with 1mol / L aqueous sodium hydroxide solution, centrifuge the mixed solution after adjusting the pH value at a speed of 8000r / min for 10min, and take the supernatant Filter to obtain spray liquid; in the mixed liquid, the mass fraction of water-soluble chitosan is 2%, the mass fraction of Pluronic F127 is 1%, the volume fraction of DMSO is 30%, and the volume fraction of ethanol is 20%;
[0069] Preparation of rece...
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