Single-component TMB coloration liquid and preparation method thereof
A color developing solution, one-component technology, used in measuring devices, instruments, scientific instruments, etc., can solve problems such as product instability, and achieve the effects of eliminating ion interference, maintaining stability, and promoting dissolution.
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[0041] The present invention also provides a preparation method for the above-mentioned one-component TMB chromogenic solution, comprising the steps of:
[0042] (1) Solution A: Take 900ml of citric acid-sodium citrate buffer solution, add 0.188-1.88g of carbamide peroxide, stir to dissolve it fully, dilute to 1L with citric acid-sodium citrate buffer solution, and seal it temporarily in the dark. live.
[0043] (2) Liquid B: Weigh 4-10g of polyvinyl alcohol, add it to 500ml of 0.1mol / L citric acid-sodium citrate buffer solution at room temperature (not exceeding 40°C) while stirring, disperse evenly and then heat up Accelerate the dissolution, and finally stir and dissolve in a 60-65°C water bath, and keep warm for 2-2.5 hours until the solution no longer contains tiny particles, which can prevent agglomeration and speed up the dissolution; add 50-100g of polyvinylpyrrolidone to make it fully Dissolve; after cooling down to room temperature, add TMB0.4-1.0g, stir to fully di...
Embodiment 1
[0048] This embodiment provides a one-component TMB chromogenic solution, which is prepared by mixing liquid A and liquid B at a volume ratio of 1:1.
[0049] Described A liquid comprises each component of following concentration:
[0050] Citric acid-sodium citrate buffer: 0.1mol / L, pH3.5-3.8
[0051] Carbamide peroxide: 2mmol / L
[0052] Described B liquid comprises each component of following concentration:
[0053] Citric acid-sodium citrate buffer: 0.1mol / L, pH3.5-3.8
[0054] Polyvinyl alcohol: 0.4% (w / v)
[0055] Polyvinylpyrrolidone: 5% (w / v)
[0056] TMB: 0.04% (w / v)
[0057] Glycerin: 1% (v / v)
[0058] The citric acid-sodium citrate buffer contains 0.07mol / L citric acid and 0.03mol / L sodium citrate, and the pH is 3.5-3.8; the polyvinyl alcohol is polyvinyl alcohol 1788; the polyvinylpyrrolidone is polyvinyl alcohol Vinylpyrrolidone K-30.
[0059] The present invention also provides a preparation method for the above-mentioned one-component TMB chromogenic solu...
Embodiment 2
[0069] This embodiment provides a one-component TMB chromogenic solution, which is prepared by mixing liquid A and liquid B at a volume ratio of 1:1.
[0070] Described A liquid comprises each component of following concentration:
[0071] Citric acid-sodium citrate buffer: 0.1mol / L, pH3.5-3.8
[0072] Carbamide peroxide: 10mmol / L
[0073] Described B liquid comprises each component of following concentration:
[0074] Citric acid-sodium citrate buffer: 0.1mol / L, pH3.5-3.8
[0075] Polyvinyl alcohol: 0.7% (w / v)
[0076] Polyvinylpyrrolidone: 7.5% (w / v)
[0077] TMB: 0.07% (w / v)
[0078] Glycerin: 1.5% (v / v)
[0079] Raw material and preparation method are with embodiment 1.
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