Sulphating catathelasma ventricosum polysaccharide preparing method

Abstract

The invention discloses a sulphating catathelasma ventricosum polysaccharide preparing method. The method includes the steps that catathelasma ventricosum is frozen, dried and ground into powder, extraction of alcohol sedimentation is conducted through ethanol water, deproteinization is conducted, small molecular weight impurity removal is conducted, and the catathelasma ventricosum polysaccharide is obtained; the method for preparing the sulphating catathelasma ventricosum polysaccharide includes the steps that 70 ml of dimethyl sulfoxide is weighed to be placed into a dry three-necked flask provided with a condensing unit, catathelasma ventricosum polysaccharide samples are added slowly to the flask while magnetic stirring is conducted, the samples are prevented from condensing into blocks, stirring is conducted for 1 hour, and a bottle plug is arranged on the flask in a covered mode; then sulfur trioxide-pyridine powder is added, the mixture is heated to 85 DEG C in a water bath mode, a condenser pipe conducts backflow, and a reaction is conducted for 3 hours; after the reaction is completed, the flask is placed into a cold bath to be cooled to the room temperature, then reaction liquid is neutralized through NaOH, absolute ethyl alcohol is added for sediment, the mixture stand still for a night, the sediment is collected in a centrifugal mode and dissolved in water for dialysis, the dialysis liquid is frozen and dried, and the sulphating catathelasma ventricosum polysaccharide is obtained.

Description

technical field [0001] The invention relates to the technical field of preparation of polysaccharides, in particular to a method for preparing sulfated polysaccharides from pine mushroom polysaccharides (sCVPs). Background technique [0002] With the growth of individual piglets and the consumption of the mother itself, the nutrients obtained by the piglets from the mother's milk in the middle and late stages of lactation cannot fully meet their growth and development needs, resulting in slow growth of the piglets, and it is also not conducive to the physical function of the sow recovery. Therefore in the modern pig industry, along with the continuous expansion of the breeding scale, people generally realize that timely weaning of piglets has become an important link in pig production. Moreover, the intestinal tract of piglets is not well-developed, and early weaning can easily lead to intestinal oxidative stress damage and then lead to digestive disorders and diarrhea, whi...

AI Technical Summary

Problems solved by technology

However, the method of sulfation of polysaccharides is widespread at this stage: the activity of sulfated polysaccharides is low, the degree of substitution of sulfated polysaccharides is low, and when the concentration of reagents and reaction time are increased, the yield of sulfated polysaccharides will be seriously affected.

At present, there is no research on the sulfation of polysaccharides from the polysaccharides of P. Inversely proportional, so the application of sulfated polysaccharides to anti-diabetic research will provide new ideas and references for the chemical modification of polysaccharides and the biological activity of polysaccharides

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