Reagent for Determination of Cholesterol in Serum High Density Lipoprotein

Abstract

The invention discloses a determination reagent of cholesterols in serum high density lipoprotein. The reagent comprises a proper amount of an antiseptic, a stabilizer, a toner, a high affinity enzyme compound, a surfactant and bovine serum albumin, and the high affinity enzyme compound is prepared through the following steps: 1, carrying out enzyme activation: selecting an enzyme, pre-culturing the enzyme, carrying out activation culture on the enzyme, and carrying out a dehydration reaction; and 2, preparing the high affinity enzyme compound: selecting the compound, and carrying out a hybrid reaction. The reagent can be used to detect the cholesterols on a fully-automatic biochemical analyzer with various models without sample centrifuge, precipitation or other complicated operations, so the detection workload is greatly reduced, the detection time is shortened, and the detection cost is reduced; and the reagent has the advantages of good specificity, high sensitivity and high detection accuracy, and compared with a detection result of a nationally recommended Friedewald formula, the result of the reagent shows that the correlation reaches 0.997 or above.

Description

technical field [0001] The invention belongs to the technical fields of medicine and biochemistry, in particular to a reagent for measuring cholesterol in serum high-density lipoprotein. Background technique [0002] Serum cholesterol concentration can be used as a marker of human lipid metabolism. High-density lipoprotein cholesterol (HDL) is the smallest lipoprotein. Compared with other lipoproteins, HDL contains the largest amount of protein (>50%), and its main apolipoproteins are APOA-I, A-II, and a small amount APO-C, E; phospholipid is its main lipid, and a small amount of cholesterol, cholesteryl ester and triglyceride. Under the action of lecithin cholesterol acyltransferase (LCAT), free cholesterol becomes cholesteryl ester, which passes through HDL Transport the free cholesterol accumulated in the tissue to the liver, reduce the concentration of free cholesterol in the plasma HDL, form a concentration gradient of cholesterol flowing from the cell membrane to t...

AI Technical Summary

Problems solved by technology

Both of the above two documents disclose the technical scheme of using high-affinity enzyme compounds as detection substances, but the preparation methods of the high-affinity enzyme compounds in these two documents are simple, and the enzymes are not activated during preparation. The reaction time of the compound is long and the reaction is not sufficient, which affects the overall performance and yield of the enzyme compound

In addition, the reports on the determination of cholesterol in high-density lipoprotein are all prepared with high-molecular-weight chemical components, and the cost of compound preparation is high and the purity is low, which will affect the test results.

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