A microfluidic chip for multi-index detection
A microfluidic chip and multi-indicator technology, applied in the direction of laboratory containers, laboratory utensils, chemical instruments and methods, etc., can solve adverse detection reactions, precise control of detection results, accurate analysis of reaction reagents, cross-contamination, and sample volume Differences and other issues, to avoid reagent contamination and mutual interference, low cost, simple structure
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Embodiment 1
[0028] Embodiment 1: microfluidic chip structure
[0029] Such as figure 1 , 2 , 3, 4, and 5: a microfluidic chip for multi-biochemical index detection, which is formed by bonding and sealing chip materials integrated with microvalve, including a gas control channel layer 100, an organic polymer Membrane 200, microfluidic channel layer 300, wherein:
[0030] The gas control channel layer 100 is provided with a sample injection hole 1, an air outlet hole 6, two pneumatic microvalves 2, 3 and the upper half of a plurality of serially connected sample liquid storage pools 4, and the upper half of the sample liquid storage pool 4 One side is an elastic film and is exposed to the air;
[0031] The microfluidic channel layer 300 includes a liquid channel 7 connecting the sample injection hole and each sample liquid storage pool, a liquid channel 8 connecting each sample liquid storage pool with the corresponding reaction pool, the lower part of the sample liquid storage pool, and...
Embodiment 2
[0038] Example 2: Microfluidic Chip Workflow
[0039] Step 1: When adding samples, the microvalve 2 is opened, the microvalve 3 is closed, the pressurized gas is passed into the microvalve air channel 13 through the valve seat 12, and the liquid channel from the liquid storage pool to the reaction pool is closed, and the sample is injected from the sample The hole 1 is injected, the sample flows into each sample reservoir 4 through the liquid channel 7, and the excess sample flows into the sample buffer pool 5;
[0040] Step 2: When all the sample liquid storage pools 4 are full, the microvalve 2 is closed, the microvalve 3 is opened, and the pressurized gas is passed into the microvalve air channel 11 through the valve seat 10, and the control of each liquid storage pool 4 is closed. The micro-valve for liquid flow in the liquid channel 7 realizes the isolation between the sample liquid storage pools 4; open the micro-valve controlling the liquid channel between each sample l...
Embodiment 3
[0041] Embodiment 3: Microfluidic chip manufacturing method
[0042] (1) Preparation of molds with microstructures on microfluidic chips
[0043] The microchannel and microstructure of the microfluidic chip were designed with computer-aided design software, and the mask plates of the gas control channel layer and the microfluidic channel layer were respectively made with a chromium plate according to the design drawing.
[0044] Use silicon wafers for photolithography and etching to make molds. First, clean the silicon wafers. After the cleaned silicon wafers are dried with nitrogen, they are placed on the coating machine, and an appropriate amount of photoresist is applied to the silicon wafers, and the coating machine is started. Spin the photoresist, place the mask plate on the silicon wafer coated with photoresist, and then put it in the photolithography machine for exposure. After developing and post-baking, use dry etching to obtain the upper gas control channel layer an...
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