Polypeptide for promoting bone formation, synthesis method and applications thereof

A synthesis method and osteogenesis technology are applied in the field of biomedicine to achieve the effect of promoting the formation of bone cells, not easy to degrade, and the synthesis method is simple

Active Publication Date: 2015-12-23
FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Polypeptide for promoting bone formation, synthesis method and applications thereof
  • Polypeptide for promoting bone formation, synthesis method and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 1. This example induces the synthesis of osteogenic polypeptide

[0024] SEQ ID NO: 1 (KIPKASSVPTELSAISTLYL).

[0025] The synthesis method of the above-mentioned polypeptide promoting bone formation is carried out according to the following steps:

[0026] (1) Using RinkAmide resin or Fmoc-Gly-WANG resin as the starting material, using Fmoc-protected amino acids as monomers, using a condensing agent (HBTU / HOBt) for peptide grafting reaction, connecting amino acids one by one, and using the last peptide chain Protected mercaptopropionic acid, after obtaining the protected resin, remove side chain protecting groups and cut peptides simultaneously;

[0027] (2) Add peptide cutting reagent (TFA / EDT / HAc / TIS / EDT) to cut peptide;

[0028] (3) using diethyl ether as a solvent, precipitating and collecting the crude polypeptide that promotes bone formation;

[0029] (4) dissolving the crude polypeptide of bone formation in water, adjusting the pH to 7.5-10.0 with ammonia wat...

Embodiment 2

[0072] 1. This example induces the synthesis of osteogenic polypeptide

[0073] SEQ ID NO: 2 (KIPAASSVPTELSAISTLYL).

[0074] The synthesis method of the above-mentioned polypeptide promoting bone formation is carried out according to the following steps:

[0075] (1) Use RinkAmide resin or Fmoc-Gly-WANG resin as the starting material, use Fmoc-protected amino acids as monomers, use condensing agent (TBTU / HOAt) for peptide reaction, connect amino acids one by one, and use the last peptide chain Protected mercaptopropionic acid, after obtaining the protected resin, remove side chain protecting groups and cut peptides simultaneously;

[0076] (2) Add peptide cutting reagent (TFA / EDT / HAc / TIS / EDT) to cut peptide;

[0077] (3) using diethyl ether as a solvent, precipitating and collecting the crude polypeptide that promotes bone formation;

[0078] (4) dissolving the crude polypeptide of bone formation in water, adjusting the pH to 7.5-10.0 with ammonia water, and collecting the...

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Abstract

The present invention belongs to the technical field of biological medicine, and discloses a polypeptide for promoting bone formation, a synthesis method and applications thereof, wherein the polypeptide has the amino acid residue sequence represented by SEQ ID NO:1 in the sequence table. According to the present invention, the calcined bone targeting binding polypeptide can promote the bone cell generation, the synthesis method is simple, the mass production can be achieved, and the synthesized polypeptide has characteristics of degradation resistance and high activity, and is especially suitable for osteoporosis treatment.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a polypeptide for promoting bone formation and its synthesis method and application. Background technique [0002] At present, through the research on the mechanism of BMP, the active sequence that plays a role in it is selected for in vitro synthesis, and the active region with targeted binding to mineral scaffolds is designed, and BMP that can be stably combined with calcined bovine bone scaffolds and released slowly -2 functional polypeptide, the synthesis cost is lower than 1% of the market price of BMP-2. In the activity comparison experiment with BMP-2 protein, the promotion effect of BMP-2 functional polypeptide on key indicators such as osteoblast proliferation and alkaline phosphatase activity is better than that of BMP-2 protein. In vivo animal evaluation is currently underway, and the main results will be available within 1-2 months. The functional po...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12N15/11C07K1/06A61K38/10A61P19/10
CPCY02P20/55
Inventor 赵彦涛朱加亮衷鸿宾侯树勋韩丽伟白玉龙
Owner FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
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