Method for determining antibody titer by using antigen single immunodiffusion
An antibody titer, unidirectional diffusion technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of deviation in measurement results, cumbersome measurement process, etc., achieve fast and accurate results, accurate measurement, and eliminate Becker's method deviation. Effect
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Embodiment 1
[0017] A method for determining antibody titer using the antigen unidirectional diffusion method in this embodiment comprises the following steps:
[0018] 1) Seal one end of a graduated glass tube with adhesive tape, the inner diameter of the glass tube is 4mm, and the outer diameter is 5.5mm;
[0019] 2) Fill the glass tube with an agarose molten solution containing the antibody to be tested. The volume of the molten agarose solution containing the antibody to be tested is 5 milliliters, and the volume of the antibody to be tested added to the molten agarose solution is 125 microliters;
[0020] 3) After the molten agarose solution is solidified, open the adhesive tape sealed at one end of the glass tube, and vertically stand the glass tube on a base with a water-absorbing material placed at the bottom;
[0021] 4) Add 0.01 ml of antigen of known concentration to the upper end of the vertical glass tube, and the concentration of the added antigen is 3 mg / ml;
[0022] 5) Aft...
Embodiment 2
[0029] A method for determining antibody titer using the antigen unidirectional diffusion method in this embodiment comprises the following steps:
[0030] 1) Seal one end of a graduated glass tube with adhesive tape, the inner diameter of the glass tube is 4mm, and the outer diameter is 5.5mm;
[0031] 2) pour the agarose molten solution containing the antibody to be tested into the glass tube of step 1), the volume of the molten agarose solution containing the antibody to be tested is 5 milliliters, and the volume of the antibody to be tested added in the molten agarose solution is 300 μL Microliter;
[0032] 3) After the molten agarose solution is solidified, open the adhesive tape sealed at one end of the glass tube, and vertically stand the glass tube on a base with a water-absorbing material placed at the bottom;
[0033] 4) Add 0.05 ml of antigen of known concentration to the upper end of the vertical glass tube, and the concentration of the added antigen is 3 mg / ml; ...
Embodiment 3
[0041] A method for determining antibody titer using the antigen unidirectional diffusion method in this embodiment comprises the following steps:
[0042] 1) Seal one end of a graduated glass tube with adhesive tape, the inner diameter of the glass tube is 4mm, and the outer diameter is 5.5mm;
[0043] 2) perfuse the agarose molten solution containing the antibody to be tested in the glass tube of step 1), the volume of the molten agarose solution containing the antibody to be tested is 4.8 milliliters, and the volume of the antibody to be tested added in the molten agarose solution is 240 Microliter;
[0044] 3) After the molten agarose solution is solidified, open the adhesive tape sealed at one end of the glass tube, and stand the glass tube vertically on a base with a water-absorbing material placed at the bottom;
[0045] 4) Add 0.03 ml of antigen of known concentration to the upper end of the vertical glass tube, and the concentration of the added antigen is 15 mg / ml; ...
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