A kind of thrombin time determination reagent and preparation method thereof
A technology of thrombin time and bovine thrombin, which is applied in the field of thrombin time measurement reagents and its preparation, can solve the problems of increasing the use and control of raw materials, lowering the glass transition temperature of the mixed solution, increasing the viscosity of the mixed solution, etc., and reducing the dosage , reduce catalysis and improve accuracy
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Embodiment 1
[0016] Weigh 6.2g Tris and 2.5g sodium chloride, add them into a glass bottle with a volume of 1.5L, add deionized water to 500mL, stir to dissolve, adjust the pH to 7.3 with dilute hydrochloric acid, add 2g bovine serum albumin, 1.4g chloride Calcium chloride, 2g of polyethylene glycol, 15g of mannitol, 10g of maltose, and 0.5g of sodium azide were thoroughly mixed, and the volume was adjusted to 1L, and bovine thrombin was added to make the bovine thrombin concentration 5U / ml. After stirring and mixing, the reagent for measuring thrombin time is obtained.
Embodiment 2
[0018] Weigh 6.2g Tris and 2.5g sodium chloride, add them into a glass bottle with a volume of 1.5L, add deionized water to 500mL, stir to dissolve, adjust the pH to 7.3 with dilute hydrochloric acid, add 1.5g bovine serum albumin, 1.4g Calcium chloride, 2g polyethylene glycol, 15g mannitol, 10g maltose, 0.5g sodium azide, 10g valine, after thorough mixing, set the volume to 1L, add bovine thrombin to make the concentration of bovine thrombin It is 5U / ml. After stirring and mixing, the thrombin time determination reagent is obtained.
Embodiment 3
[0020] Weigh 6.2g Tris and 2.5g sodium chloride, add them into a glass bottle with a volume of 1.5L, add deionized water to 500mL, stir to dissolve, adjust the pH to 7.3 with dilute hydrochloric acid, add 1.5g bovine serum albumin, 1.4g Calcium chloride, 2g polyethylene glycol, 15g mannitol, 10g maltose, 10g valine, 2mM coumaric acid, after thorough mixing, set the volume to 1L, add bovine thrombin to make the bovine thrombin concentration 5U / ml, after stirring and mixing, the thrombin time determination reagent is obtained.
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