Determination method of lactic acid content in feed acidifier

Abstract

The invention relates to a method for detecting the content of lactic acid in a feed acidifier. The detecting method comprises the steps of preparing a sample to be detected, adding the sample to be detected to strong base to depolymerize lactic anhydride and conducting dilution and filtration in sequence, conducting sample detection, and conducting result analysis. The method is used for liquid chromatogram quantitative analysis of the content of lactic acid in a mixed type feed additive, especially an acidifier, with silicon dioxide and / or mountain flour as a carrier; dissociation of polylactic acid is conducted firstly with strong base, then pH is regulated to guarantee sufficient protonation under the acidic condition, volume setting is conducted, filtration is conducted with an organic filter membrane, and finally filtrate is injected into the sample for detection. According to the method, the chemical properties of lactic acid are utilized, special pretreatment is conducted, the accuracy and precision of a detection result are improved greatly, operation is easy, the reagent adopted is safe and free of toxicity, the safety of detection staff is high, results are accurate and stable, and adopted equipment are common and easy to obtain. The method is an ideal lactic acid liquid chromatogram detection method and is suitable for large-scale application and popularization.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to the detection of mixed feed additive components, in particular to a detection method for lactic acid content in a feed acidifier. Background technique [0002] Lactic acid, scientific name 2-hydroxypropionic acid, molecular formula C 3 h 6 o 3 , is a carboxylic acid, odorless, hygroscopic, miscible with water, ethanol, glycerin, insoluble in chloroform, carbon disulfide and petroleum ether. In the field of feed additives, lactic acid can be used to equip feed mixed acidifiers to replace feed antibiotics, ensure the health of livestock and poultry, promote growth and production, and can also inhibit harmful bacteria, promote the growth of beneficial bacteria, and regulate the gastrointestinal tract. pH, plays a dual role of antibiotics and bacteriobiotics. When equipped with a feed mixed acidifier, lactic acid and other organic acids are usually added to carrier...

AI Technical Summary

Problems solved by technology

[0012] The purpose of the present invention is to overcome the shortcoming of low detection accuracy due to the insufficient depolymerization of lactic acid polymers into lactic acid monomers when lactic acid is detected in the mixed acidifier of the above prior art, and to provide a sample that can be realized Fully depolymerized and then detected by liquid chromatography to ensure the high accuracy of the results of the detection method of lactic acid content in feed acidifier

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