An anaerobic microorganism enrichment culture device

Abstract

The invention relates to an anaerobic microbe enrichment culture device. The anaerobic microbe enrichment culture device is characterized in that the enrichment culture device comprises a methane generator (1), an air distributing device (3), air distributing pipes (5), an air discharging device (6) and a plurality of DAMO enrichment containers (7). The methane generator (1) is communicated with the air distributing device (3). The air distributing device is communicated with the plurality of DAMO enrichment containers (7) through the plurality of air distributing pipes (5). A motor (4) is arranged above the methane generator (1). The motor can drive propellers extending into the methane generator (1) to rotate to gather generated methane gas. The air discharging device (6) comprises an annular water tank (601), a cover cap (602) and a gas outlet (603). The cover cap (602) covers the annular water tank (601). The lower end of the cover cap is under the liquid level. When the gas pressure in the DAMO enrichment containers (7) is too high, the cover cap (602) is jacked up by the gas pressure, and gas discharging and pressure release are carried out.

Description

technical field [0001] The invention relates to a device for enriching and cultivating anaerobic microorganisms, which belongs to the technical field of enriching and cultivating denitrifying methane anaerobic oxidizing bacteria. Background technique [0002] Denitrification and denitrification is one of the main processes in the field of sewage treatment. It is traditionally believed that in the denitrification process, anaerobic denitrification bacteria use nitrogen oxides as electron acceptors and organic carbon sources such as glucose and lactose as electron donors. However, studies in recent years have shown that in the process of denitrification, a class of microorganisms can use methane as an electron donor and NO3- or NO2- as an electron donor to simultaneously generate N2 and CO2 under anaerobic conditions. These microorganisms are called denitrifying anaerobic methane oxidizers (DAMO). Methane is one of the main greenhouse gases, and methane is the main product o...

AI Technical Summary

Problems solved by technology

Moreover, the price of pure methane gas is high, and the solubility of methane is extremely low. Most of the methane passed in escapes into the atmosphere, and the utilization rate is low, causing serious waste and air pollution.

Therefore, the existing technology directly uses high-pressure pure methane gas to enrich and cultivate DAMO, which is not only costly, but also has great potential safety hazards.

On the other hand, most of the existing DAMO enrichment systems have only one enrichment container, so it is difficult to set parallel and control during the experiment, let alone set the experimental gradient, and the experiment lacks reproducibility and scientificity

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