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A screening kit for female yang-deficiency infertility

A technology of infertility and reagents, applied in the direction of microbial measurement/testing, biochemical equipment and methods, etc., can solve problems such as no testisin, and achieve good results in clinical application prospects

Active Publication Date: 2019-01-04
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There is no report on the relationship between testisin and yang deficiency infertility

Method used

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  • A screening kit for female yang-deficiency infertility
  • A screening kit for female yang-deficiency infertility
  • A screening kit for female yang-deficiency infertility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 The relationship between the expression level of testisin gene and female yang deficiency constitution infertility

[0024] 1. Materials and methods

[0025] 1.1 Experimental materials

[0026] 1.1.1 Main experimental instruments

[0027] Quantitative PCR instrument MJ Research, PTC-200

[0028] UV spectrophotometer BECKMAN

[0029] C1000 Thermal Cycler BIO-RAD

[0030] 1.1.2 Main experimental materials and reagents

[0031]

[0032] 1.1.3 Reagent preparation:

[0033] (1) PBS buffer (g / 1,000mL):

[0034] NaCl

8.0

KCl

0.2

Na 2 HPO 4 12H 2 o

1.54

K H 2 PO 4

0.2

[0035] Add DEPC-treated RNase-Free water, the pH value is about 7.3, dilute to 1000mL, and autoclave.

[0036] (2) Red blood cell lysate:

[0037] drug

Final concentration

molecular weight

required amount

KHCO 3

10mM

100

1g

NH 4 Cl

155mM

53.49

8.29g

EDTA

0.1mM

3...

Embodiment 2

[0134] Embodiment 2 The composition of kit of the present invention and the detection of sample to be tested

[0135] 1. RT-PCR detection kit

[0136] 1. The composition of the kit

[0137] Reverse transcription reagent (50 people):

[0138] components

volume

5x iScript reaction mix

200ul

iScript reverse transcriptase

50ul

Nuclease-free water

350ul

RNA sample

400ul

Total Volume

1ml

[0139] Amplification and detection reagents (50 persons):

[0140] components

concentration

volume

MasterMix

500ul

Primer pair for the target gene testisin

5μM

50μl

Primer pair for β-Actin

5μM

50μl

RNase-free water

2ml

PCR reaction plate

2 pieces of 384-well plate

[0141] The primer pair of the target gene testisin (shown in SEQ ID NO.2-3):

[0142] F:5-ATAGTGGGTGGCGATGA-3

[0143] R:5-TTGGTAACGGTTGGAAT-3

[0144] Primer pair for ...

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PUM

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Abstract

The invention discloses a screening kit for female yang deficiency constitution infertility. The screening kit comprises an optional reagent used for testing testisin gene expression level. The invention also discloses application of the reagent used for testing testisin gene expression level in preparing the reagent for screening female yang deficiency constitution infertility. The kit can test the testisin gene expression level to determine the risk of female yang deficiency constitution infertility suffered by person to be checked, can be used for clinical auxiliary diagnosis of female yang deficiency constitution infertility, can provide effective basis for relevant treatment or decision of patients, and has a good clinical prospect.

Description

technical field [0001] The invention relates to a screening kit for female yang-deficiency infertility. Background technique [0002] The medical definition of infertility is not taking any contraceptive measures for one year, having normal sexual life without successful pregnancy. Mainly divided into primary infertility and secondary infertility. Primary infertility refers to never conceived; secondary infertility refers to infertility after being pregnant. By this strict definition, infertility is a common problem, affecting at least 10% to 15% of couples of reproductive age. The causes of infertility are divided into male infertility and female infertility. [0003] Yang deficiency is the common pathological mechanism of yang deficiency syndrome and yang deficiency constitution. Its main pathogenesis is the deficiency and decline of yang qi in the body, and the main manifestations are cold symptoms such as cold limbs and cold body. Yang-deficiency constitution is a co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883
Inventor 陆华高小平刘志斌刘芊辰梁丽梅
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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