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Screening kit for female yang deficiency constitution infertility

A kit and infertility technology, which can be used in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as unseen testisin, and achieve good clinical application prospects.

Active Publication Date: 2016-01-20
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There is no report on the relationship between testisin and yang deficiency infertility

Method used

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  • Screening kit for female yang deficiency constitution infertility
  • Screening kit for female yang deficiency constitution infertility
  • Screening kit for female yang deficiency constitution infertility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The relationship between the expression level of embodiment 1 testisin gene and women's infertility due to yang deficiency

[0024] 1. Materials and methods

[0025] 1.1 Experimental materials

[0026] 1.1.1 Main experimental instruments

[0027] Quantitative PCR instrument MJResearch, PTC-200

[0028] UV spectrophotometer BECKMAN

[0029] C1000 Thermal Cycler BIO-RAD

[0030] 1.1.2 Main experimental materials and reagents

[0031]

[0032] 1.1.3 Reagent preparation:

[0033] (1) PBS buffer (g / 1,000mL):

[0034] NaCl 8.0 KCl 0.2 Na 2 HPO 4 12H 2 o 1.54 K H 2 PO 4 0.2

[0035] Add DEPC-treated RNase-Free water, the pH value is about 7.3, dilute to 1000mL, and autoclave.

[0036] (2) Red blood cell lysate:

[0037] drug Final concentration molecular weight required amount KHCO 3 10mM 100 1g NH 4 Cl 155mM 53.49 8.29g EDTA 0.1mM 372.24 0.5M 100μL

[0038] Add DEPC-treated ...

Embodiment 2

[0134] Embodiment 2 The composition of kit of the present invention and the detection of sample to be tested

[0135] 1. RT-PCR detection kit

[0136] 1. The composition of the kit

[0137] Reverse transcription reagent (50 people):

[0138] components volume 5x iScript reaction mix 200ul iScript reverse transcriptase 50ul Nuclease-free water 350ul RNA sample 400ul Total Volume 1ml

[0139] Amplification and detection reagents (50 persons):

[0140] components concentration volume MasterMix

500ul Primer pair for the target gene testisin 5μM 50μl Primer pair for β-Actin 5μM 50μl RNase-free water

2ml PCR reaction plate

2 pieces of 384-well plate

[0141] The primer pair of the target gene testisin (shown in SEQ ID NO.2-3):

[0142] F:5-ATAGTGGGTGGCGATGA-3

[0143] R:5-TTGGTAACGGTTGGAAT-3

[0144] The primer pair of β-Actin (shown in SEQ ID NO.4~5):

[0145] F:5...

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PUM

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Abstract

The invention discloses a screening kit for female yang deficiency constitution infertility. The screening kit comprises an optional reagent used for testing testisin gene expression level. The invention also discloses application of the reagent used for testing testisin gene expression level in preparing the reagent for screening female yang deficiency constitution infertility. The kit can test the testisin gene expression level to determine the risk of female yang deficiency constitution infertility suffered by person to be checked, can be used for clinical auxiliary diagnosis of female yang deficiency constitution infertility, can provide effective basis for relevant treatment or decision of patients, and has a good clinical prospect.

Description

technical field [0001] The invention relates to a screening kit for female yang-deficiency infertility. Background technique [0002] The medical definition of infertility is not taking any contraceptive measures for one year, having normal sexual life without successful pregnancy. Mainly divided into primary infertility and secondary infertility. Primary infertility refers to never conceived; secondary infertility refers to infertility after being pregnant. By this strict definition, infertility is a common problem, affecting at least 10% to 15% of couples of reproductive age. The causes of infertility are divided into male infertility and female infertility. [0003] Yang deficiency is the common pathological mechanism of yang deficiency syndrome and yang deficiency constitution. Its main pathogenesis is the deficiency and decline of yang qi in the body, and the main manifestations are cold symptoms such as cold limbs and cold body. Yang-deficiency constitution is a co...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6883
Inventor 陆华高小平刘志斌刘芊辰梁丽梅
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE